Route design
Chemical synthesis method (solid phase synthesis SPPS, liquid phase synthesis): for short peptides (biasane ≤50 asam amino), need to select protection group strategy, reagen kopling, lsp.
Biosynthesis (recombinant DNA technology): expression of long peptides or complex modified peptides by microorganisms or cells, requires optimization of host selection (contone. E. coli, ragi) and culture conditions..
Process optimization
Synthesis/expression optimization: increase yield (contone. suhu, pH, catalyst concentration).
Purification process: chromatography (reversed-phase HPLC, ion exchange) is key, need to optimize elution conditions.
Kontrol impurity: remove missing peptides, mismatched sequences, oxidation products, lsp. to ensure purity (biasane >98%).