Custom Synthesis and Ordering Questions and Answers

Cost, lead time, sequence design, solubility, storage and reconstitution for custom orders.

171 deduplicated search questions in this category, answered by 8 in-depth guides.

In-depth answers

How Do I Order Custom Peptides? To order a custom peptide you provide the exact sequence, the quantity, the purity you need and any modifications or labels. The supplier reviews the sequence for synthesis risk, confirms the specification and price in a written quote, then proceeds to synthesis, purification, quality control and lyophilisation. Routine orders are typically delivered two to four weeks after the purchase order. How Much Does Custom Peptide Synthesis Cost? Custom peptide prices are quoted per project rather than per catalogue item, and no single per-amino-acid rate is reliable. The price reflects the number of couplings, reagent consumption, purification losses, analytical work and documentation. As a working rule, longer sequences, higher purity, difficult motifs and smaller batches all raise the per-milligram cost, while larger batches lower it but not proportionally. How Long Does Peptide Synthesis Take? Routine custom peptides typically take two to four weeks from purchase order to dispatch. Short, unmodified sequences at moderate purity are usually at the fast end. Long chains, difficult motifs, multiple modifications, high purity targets and large batches take longer, and expedited slots are available subject to production capacity. Shipping time is additional and depends on destination and customs. How Long Can a Custom Peptide Be? Chemical synthesis routinely delivers peptides of 10 to 40 residues, and experienced laboratories can reach 70 to 100 residues for favourable sequences. Beyond that, yield and purity fall sharply because incomplete coupling accumulates at every step. Long or difficult targets are usually handled by fragment condensation, native chemical ligation or recombinant expression rather than single-chain solid-phase synthesis. How Do You Dissolve a Peptide? Peptide solubility is driven by charge, hydrophobicity and isoelectric point. Start with the most polar solvent likely to work, usually water, then move to dilute acetic acid or ammonium hydroxide to shift the pH away from the pI, then acetonitrile-water, DMSO or HFIP for hydrophobic chains. Screen small aliquots in that order, note what dissolves, and swirl rather than vortex. How Should Peptides Be Stored? Store lyophilized peptide at -20 C, desiccated and protected from light, where it is typically stable for years. Once reconstituted, keep solutions at 4 C for short-term use and freeze single-use aliquots at -20 C or -80 C for longer storage. Avoid repeated freeze-thaw cycles, and choose bacteriostatic water only when its preservative is compatible with the assay. How Do You Reconstitute a Lyophilized Peptide? Reconstitution means adding a solvent to lyophilized peptide so that it returns to solution. Calculate the target concentration from the peptide mass, add the solvent slowly down the vial wall rather than onto the powder, swirl gently and inspect for clarity. Record the lot, solvent, concentration and date. The procedure is for laboratory handling of research material only. Custom vs Catalogue Peptides: Which Should You Choose? Catalogue peptides are pre-synthesized, validated lots available quickly and at lower cost, but limited to published sequences and fixed specifications. Custom peptides are made to your exact sequence, scale, purity and modification. Choose catalogue when a standard sequence exists; choose custom when the sequence is proprietary, modified, unusually pure or must match a specific lot specification.

All custom synthesis and ordering questions

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