{"id":6901,"date":"2025-04-28T16:07:46","date_gmt":"2025-04-28T08:07:46","guid":{"rendered":"https:\/\/molchanges.com\/?page_id=6901"},"modified":"2025-04-28T16:07:46","modified_gmt":"2025-04-28T08:07:46","slug":"peptide-faq","status":"publish","type":"page","link":"https:\/\/molchanges.com\/zh\/peptide-faq","title":{"rendered":"Peptide FAQ"},"content":{"rendered":"\t\t
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Set your favorite Elementor FAQ style to help users get the exact information.<\/span><\/p>\n\t\t\t\t<\/div>\n\t\t\t<\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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How to store peptides?<\/h2>\n <\/div>\n
\n 1. Raw peptides
\nFor long term storage of peptides, we recommend storing peptides as a solid powder, as peptides in the raw powder state can be stored at -20 \u00b0C or lower to minimise degradation. We also recommend that custom peptides are rechecked for purity every 2 years.

\n\n2. Peptides in solution \nPeptides in solution are less stable and prone to degradation. Aqueous solutions used to dissolve peptides should be sterile and pure.\nIn solution, a certain amount of peptide may be degraded, depending on the amino acids present in the sequence.
\nA few examples are shown below:
\n- Peptides containing methionine, cysteine or tryptophan residues should have a limited storage time in solution due to oxidation. These peptides should be dissolved in an oxygen-free solvent.

\n- Glutamine and asparagine can be deamidated to glutamic acid and aspartic acid, respectively.\n- Cysteine can undergo oxidative cyclisation to form Cys-Cys disulphide bonds (either intra- or inter-disulphide bonds can be formed).

\n- Charged residues (Asp, Glu, Lys, Arg, His) are hygroscopic (absorbing water from moisture in the air) and can easily form a viscous, clear oil, a physical change that may not affect the properties of the peptide.

\nIt is recommended that such peptides are first lyophilised to remove traces of water, degassed with nitrogen to ensure that there is no water vapour in the vial, and then quickly capped.

\nTo prevent damage from repeated freeze-thaw cycles, we recommend that users only dissolve the amount of peptide required for the current experiment. Any excess solution should be stored at \u2265 -20 \u00b0C until needed.

\n\nAvoid humidity \nAs humidity can greatly reduce the long-term stability of peptides, we recommend that peptides should be equilibrated to room temperature in a desiccator before opening vials. After peptide dispensing, the remaining peptide in the tube should be gently purged with anhydrous nitrogen, the container capped, sealed with a sealing film, and stored at -20 \u00b0C. <\/div>\n <\/div>\n