{"id":6916,"date":"2025-04-28T16:08:27","date_gmt":"2025-04-28T08:08:27","guid":{"rendered":"https:\/\/molchanges.com\/?page_id=6916"},"modified":"2025-04-28T16:08:27","modified_gmt":"2025-04-28T08:08:27","slug":"custom-peptide-synthesis-2","status":"publish","type":"page","link":"https:\/\/molchanges.com\/zh\/custom-peptide-synthesis-2","title":{"rendered":"\u591a\u80bd\u4fee\u9970\u6837\u677f"},"content":{"rendered":"
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\u5728\u6b64\u5904\u6dfb\u52a0\u6807\u9898\u6587\u672c<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\n\n \u8367\u5149\u67d3\u6599\u6807\u8bb0\u80bd\n \n <\/h2>\n
\n \u6211\u4eec\u5f88\u9ad8\u5174\u80fd\u63d0\u4f9b\u8303\u56f4\u5e7f\u6cdb\u7684\u4f18\u8d28\u8367\u5149\u67d3\u6599\uff0c\u8fd9\u4e9b\u67d3\u6599\u8de8\u8d8a\u6574\u4e2a\u53ef\u89c1\u5149\u548c\u8fd1\u7ea2\u5916\u5149\u8c31\uff0c\u53ef\u7528\u4e8e\u5c06\u5176\u52a0\u5165\u60a8\u5b9a\u5236\u7684\u591a\u80bd\u4e2d\u3002\n\n\n <\/div>\n <\/div>\n\n
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\n \n \u67e5\u770b\u6211\u4eec\u7684\u5149\u8c31\u8303\u56f4 <\/a>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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\u6211\u4eec\u7684\u62a5\u4ef7<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\u80bd\u53ef\u4ee5\u88ab\u6807\u8bb0\u4e3a\u00a0\u5355\u67d3\u6216\u591a\u67d3\u00a0<\/strong>\u5728\u80bd\u7684 N-\u7aef\u3001C-\u7aef\u6216\u5185\u90e8\uff0c\u5e94\u7528\u4e8e\u86cb\u767d\u00a0\u4e92\u52a8\u7814\u7a76<\/strong>\u8ffd\u8e2a\u751f\u7269\u5206\u5b50\u7684\u5b58\u5728\u3002\u00a0\u7ec6\u80de\u3001\u9176\u6d3b\u6027\u6d4b\u5b9a<\/strong>\u7b49\u7b49\u3002
\u5173\u4e8e\u7528\u67d3\u6599\u548c\u6dec\u706b\u5242\u6807\u8bb0\u7684\u80bd\uff0c\u8bf7\u53c2\u9605\u6211\u4eec\u7684\u00a0FRET \u80bd<\/a>\u00a0page.<\/span>

<\/p>

\u56fe<\/strong>\u00a0HeLa \u7ec6\u80de\u5bf9 CPP \u7684\u5438\u6536\u3002\u7528\u542b\u6709 10 uM FITC-LC-Antennapedia\u3001cat# AS-24175\u3001AS-24176 \u7684 OptiMEM \u57f9\u517b\u57fa\u57f9\u517b HeLa \u7ec6\u80de 1 \u5c0f\u65f6\uff0c\u7136\u540e\u6e05\u6d17\u5e76\u7528\u8367\u5149\u663e\u5fae\u955c\u5206\u6790\u3002<\/small><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\"\"\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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\u6211\u4eec\u7684\u8367\u5149\u67d3\u6599\u6a2a\u8de8\u6574\u4e2a\u53ef\u89c1\u5149\u8c31\u548c\u8fd1\u7ea2\u5916\u5149\u8c31<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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HiLyte\u2122 \u8367\u5149\u67d3\u6599\uff08\u5bf9 pH \u503c\u4e0d\u654f\u611f\uff09<\/h3>

HiLyte\u2122 Fluor dyes are ideal for applications at high or low pH (11). These dyes also exhibit higher sensitivities, owing to their enhanced intensity and photostability.<\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\u540d\u79f0<\/th>Abs\/Em<\/th>\u5e94\u7528<\/th><\/tr>
HiLyte<\/span>\u2122<\/sup> \u6c1f 488<\/span><\/th>-<\/span><\/td>497\/525 \u7eb3\u7c73<\/td>FRET \u5206\u6790\u3001\u8ffd\u8e2a\u6216\u76f8\u4e92\u4f5c\u7528\u7814\u7a76<\/td><\/tr>
HiLyte<\/span>\u2122<\/sup> \u6c1f 555<\/span><\/th>-<\/span><\/td>550\/566 \u7eb3\u7c73<\/td>FRET\uff0c\u6807\u8bb0<\/td><\/tr>
HiLyte<\/span>\u2122<\/sup> \u6c1f 647<\/span><\/th>-<\/span><\/td>650\/675 \u7eb3\u7c73<\/td>FRET\/TR-FRET\uff0c\u6807\u8bb0<\/td><\/tr>
HiLyte<\/span>\u2122<\/sup> \u6c1f 750<\/span><\/th>-<\/span><\/td>753\/778 \u7eb3\u7c73<\/td>\u6210\u50cf<\/td><\/tr><\/tbody><\/table>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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\u53e4\u5178\u67d3\u6599<\/h3>

\u5f53\u5e94\u7528\u7684\u64cd\u4f5c pH \u503c\u8303\u56f4\u7075\u6d3b\u65f6\uff0cFAM\u3001TAMRA \u548c CyLyte Fluor \u67d3\u6599\uff08Cy Dyes \u7c7b\u4f3c\u7269\uff09\u7b49\u4f20\u7edf\u67d3\u6599\u662f\u6781\u5177\u6210\u672c\u6548\u76ca\u7684\u9009\u62e9\u3002<\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\t\t\t\t\t\t\n\n\n\n\n\n\n\n\n\n\n\n
\u540d\u79f0<\/th>\nAbs\/Em<\/th>\n\u5e94\u7528<\/th>\n<\/tr>\n
Mca<\/th>\n-<\/span><\/td>\n325\/393 \u7eb3\u7c73<\/td>\nFRET<\/td>\n<\/tr>\n
EDANS<\/th>\n-<\/span><\/td>\n335\/493 \u7eb3\u7c73<\/td>\nFRET<\/td>\n<\/tr>\n
FAM<\/th>\n-<\/span><\/td>\n492\/518 \u7eb3\u7c73<\/td>\nFRET\uff0c\u6807\u8bb0<\/td>\n<\/tr>\n
FITC<\/th>\n-<\/span><\/td>\n494\/519 \u7eb3\u7c73<\/td>\n\u6807\u7b7e<\/td>\n<\/tr>\n
CyLyte Fluor 3<\/th>\n-<\/span><\/td>\n550\/564 \u7eb3\u7c73<\/td>\nFRET\uff0c\u6807\u8bb0<\/td>\n<\/tr>\n
\u5854\u59c6\u62c9<\/th>\n-<\/span><\/td>\n541\/568 \u7eb3\u7c73<\/td>\nFRET\uff0c\u6807\u8bb0<\/td>\n<\/tr>\n
\u7f57\u514b\u65af<\/th>\n-<\/span><\/td>\n568\/591 \u7eb3\u7c73<\/td>\nFRET\uff0c\u6807\u8bb0<\/td>\n<\/tr>\n
CyLyte Fluor 5<\/th>\n-<\/span><\/td>\n648\/663 \u7eb3\u7c73<\/td>\nFRET\/TR-FRET\uff0c\u6807\u8bb0<\/td>\n<\/tr>\n
CyLyte Fluor 7<\/th>\n-<\/span><\/td>\n750\/773 \u7eb3\u7c73<\/td>\nFRET\uff0c\u6807\u8bb0<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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\u8367\u5149\u5e95\u7269<\/h3>

\u53d1\u8367\u5149\u5e95\u7269\u4e0d\u9700\u8981\u6dec\u706d\u5242\uff0c\u5b83\u542b\u6709 C \u7aef\u67d3\u6599\uff0c\u5728\u4ece\u80bd\u4e0a\u88c2\u89e3\u4e4b\u524d\u4e0d\u4f1a\u53d1\u51fa\u8367\u5149\uff08\u67d3\u6599\u7684\u8367\u5149\u5f62\u5f0f\u88ab\u91ca\u653e\u51fa\u6765\uff09\u3002<\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\u540d\u79f0<\/th>\nAbs\/Em<\/th>\n\u5e94\u7528<\/th>\n<\/tr>\n
AMC<\/th>\n-<\/span><\/td>\n351\/430 \u7eb3\u7c73<\/td>\n\u542b\u6c1f\u5e95\u7269<\/td>\n<\/tr>\n
\u4e9a\u8db3\u8054<\/th>\n-<\/span><\/td>\n382\/480 \u7eb3\u7c73<\/td>\n\u542b\u6c1f\u5e95\u7269<\/td>\n<\/tr>\n
Rh110<\/th>\n-<\/span><\/td>\n501\/527 \u7eb3\u7c73<\/td>\n\u542b\u6c1f\u5e95\u7269<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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\u7279\u8272\u5f15\u6587<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\u5229\u7528\u8367\u5149\u5404\u5411\u5f02\u6027\u6d4b\u5b9a\u6cd5\u6d4b\u91cf\u4e73\u7403\u83cc\u5be1\u80bd\u7ed3\u5408\u86cb\u767d A \u4e0e\u591a\u80bd\u7ed3\u5408\u7684 pH \u503c\u548c\u6e29\u5ea6\u4f9d\u8d56\u6027\u3002<\/h5>

Stevie Norcross\u3001Ashwin Sunderraj\u3001Mathew Tantama<\/p>

ACS Omega 2019, 4, 2812-2822, Feb 2006 2019 DOI: 10.1021\/acsomega.8b02427<\/em><\/small><\/p>

\u6765\u81ea AnaSpec\uff1a\u5408\u6210\u4e86\u78fa\u80fa 101 \u6807\u8bb0\u7684\u7f13\u6fc0\u80bd\uff08SR101-RPPGFSPFR \u548c RPPGFSPFRK-SR101\uff09\uff0c\u5e76\u7ecf HPLC \u7eaf\u5316\uff08\u7eaf\u5ea6\u5927\u4e8e 90%\uff09\u3002<\/p>

\u6dc0\u7c89\u6837\u86cb\u767d-\u03b2-\u94dc\u590d\u5408\u7269\u7684\u6c27\u5316\u8fd8\u539f\u52a8\u529b\u5b66\u53ca\u5176\u751f\u7269\u5b66\u610f\u4e49<\/h5>

Paul Girvan\u3001Xiangyu Teng\u3001Nicholas J. Brooks\u3001Geoffrey S. Baldwin \u548c Liming Ying<\/p>

Biochemistry 2018, 57, 6228-6233 DOI : 10.1021\/acs.biochem.8b00133<\/em><\/small><\/p>

\u6765\u81ea AnaSpec\uff1a\u542b\u6709\u91d1\u5c5e\u7ed3\u5408\u6b8b\u57fa\u7684\u622a\u77ed\u8367\u5149\u6807\u8bb0\u6dc0\u7c89\u6837\u86cb\u767d-\u03b2\u80bd\uff0c\u7528\u4e8e\u7814\u7a76\u5176\u91d1\u5c5e\u76f8\u4e92\u4f5c\u7528\u7279\u6027\u548c\u52a8\u529b\u5b66\u3002<\/p>

\u5728\u80bd\u529f\u80fd\u5316\u805a\u82ef\u4e59\u70ef\u8868\u9762\u5206\u79bb\u548c\u6269\u589e\u5916\u5468\u8840\u4e2d\u7684\u5185\u76ae\u7956\u7ec6\u80de<\/h5>

Mohamed A. Elkhodiry, Mari\u00e8ve D. Boulanger, Omar Bashth, Jean-Fran\u00e7ois Tanguay, Ga\u00e9tan Laroche, Corinne A. Hoesli<\/p>

\u751f\u7269\u6280\u672f\u4e0e\u751f\u7269\u5de5\u7a0b\u300b2019\uff0c116\uff0c10\uff0c2598-2609 DOI\uff1a10.1002\/bit.27107<\/em><\/small><\/p>

\u6765\u81ea AnaSpec\uff1a\u5408\u6210\u5e76 HPLC \u7eaf\u5316\u4e86\u542b\u8367\u5149\u56e2\u7684 RGD \u6807\u8bb0\u80bd\uff0c\u79f0\u4e3a RGD-TAMRA\uff08\u7eaf\u5ea6 >90%\uff09<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\u76f8\u5173\u4ea7\u54c1\u548c\u670d\u52a1<\/h2><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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\u5b9a\u5236\u591a\u80bd\u5408\u6210<\/h3>

\u6211\u4eec\u63d0\u4f9b\u7814\u7a76\u7ea7\u8367\u5149\u67d3\u6599\u6807\u8bb0\u80bd\u6216\u5173\u952e\u539f\u6750\u6599\u3002<\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\u7d22\u53d6\u62a5\u4ef7<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

Add Your Heading Text Here Fluorescent Dye-Labeled Peptides We are pleased to offer a broad range of premium fluorescent dyes that span the whole visible and near infrared spectrum, for incorporation into your custom peptides. View our spectral range Our Offer A peptide can be labeled with a\u00a0single or multiple dye(s)\u00a0at the peptide N-terminus, C-terminus, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-6916","page","type-page","status-publish","hentry"],"blocksy_meta":[],"_links":{"self":[{"href":"https:\/\/molchanges.com\/zh\/wp-json\/wp\/v2\/pages\/6916","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/molchanges.com\/zh\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/molchanges.com\/zh\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/molchanges.com\/zh\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/molchanges.com\/zh\/wp-json\/wp\/v2\/comments?post=6916"}],"version-history":[{"count":0,"href":"https:\/\/molchanges.com\/zh\/wp-json\/wp\/v2\/pages\/6916\/revisions"}],"wp:attachment":[{"href":"https:\/\/molchanges.com\/zh\/wp-json\/wp\/v2\/media?parent=6916"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}