Imitapo yolwazi ye-peptide esuselwe ku-Proteome yokucaciswa okujulile kwephrofayela ye-N-terminal modification reagents

Amaprotheni kanye ne-peptide N termini yizimpokophelo ezibalulekile zokuguqulwa okukhethiwe ngama-reagents e-chemoproteomics namathuluzi we-bioconjugation. I-N-terminal α-amine yenzeka kanye kuphela kuchungechunge lwe-polypeptide ngayinye, okwenza kube ithagethi ekhangayo ye-protein bioconjugation. Kumaseli, I-N termini entsha ingakhiqizwa i-proteolytic cleavage futhi ithathwe ama-N-terminal modification reaagents avumela ukuhlonza okubanzi kwe-proteome kwama-protease substrates nge-tandem mass spectrometry (I-LC-MS/MS). Ukuqonda ukucaciswa kokulandelana kwe-N-terminal kwama-reagents okushintsha kubalulekile kulolu hlelo lokusebenza ngalunye.. Amalabhulali e-peptide asuselwa ku-Proteome ahlanganiswe ne-LC-MS/MS angamathuluzi anamandla okwenza iphrofayela okucacile kokulandelana kwama-N-terminal modification reagents.. Lemitapo yolwazi ihluke kakhulu, kanye ne-LC-MS/MS inika amandla ukuhlaziya ukusebenza kahle kokuguqulwa kwamashumi ezinkulungwane zokulandelana ocwaningweni olulodwa.. Imitapo yolwazi ye-peptide esuselwe ku-Proteome iyithuluzi elinamandla lokufaka iphrofayela imininingwane yokulandelana kwe-enzymatic ne-chemical peptide reagents.. I-Subtiligase ne-2-pyridinecarboxaldehyde (2I-PCA) ama-reagents amabili akhelwe ukuguqulwa okukhethiwe kwe-N-terminal peptide futhi angacwaningwa kusetshenziswa imitapo yolwazi ye-peptide etholakala kwi-proteome. Le nqubo yomthetho iveza izinyathelo zokukhiqiza amalabhulali e-peptide asuselwa ku-N-terminally ahlukene asuselwa ku-proteome kanye nokusebenzisa lawa mamitapo ukuze afake iphrofayili yokucaciswa kwama-N-terminal modification reagents.. Sichaza kabanzi ngezinyathelo zokufaka imininingwane ngemininingwane ye-2-pyridinecarboxaldehyde, i-reagent yokuguqulwa kwamakhemikhali, kanye ne-subtiligase, i-reagent ye-enzymatic yokuguqulwa. Lawa maphrothokholi angashintshwa kalula kwezinye izindawo ze-proteome namanye ama-N-terminal peptide reagents..