Route design
Chemical synthesis method (solid phase synthesis SPPS, liquid phase synthesis): for short peptides (matetika ≤50 asidra amino), need to select protection group strategy, coupling reagents, sns.
Biosynthesis (recombinant DNA technology): expression of long peptides or complex modified peptides by microorganisms or cells, requires optimization of host selection (oh. f. coli, masirasira) and culture conditions..
Process optimization
Synthesis/expression optimization: increase yield (oh. hafanana, pH, catalyst concentration).
Purification process: chromatography (reversed-phase HPLC, ion exchange) is key, need to optimize elution conditions.
Impurity control: remove missing peptides, mismatched sequences, vokatra oxidation, sns. to ensure purity (matetika >98%).