Liphetoho tsa Peptide
Peptide ea cyclic
Cyclic Peptide ke eng?
Peptide cyclization ho theha li-peptide tsa cyclic kapa li-peptide tse tsamaeang ka libaesekele ke leano le sebelisoang khafetsa bakeng sa nts'etsopele ea li-peptide tse nang le botsitso bo ntlafalitsoeng ha li bapisoa le li-analogue tsa tsona tse tšoanang.. Li-peptide tsa cyclic li ipakile li thusa lits'ebetsong tse 'maloa, ho kenyelletsa le ho etsisa meaho ea bobeli ea protheine, joalo ka li-loops tsa protheine, le optimization ea li-peptide ligands ka ho eketseha ha matla a tlamang, kgetho, le botsitso bo matlafetseng ba protease.
Litlhophiso tsa libaesekele
Mekhoa ea mantlha ea ligation ea ho kopanya li-peptide tsa lactam cyclic ke hlooho-to-mohatla, ketane ka mahlakoreng, hlooho-to-hlakoreng-ketane, le lehlakore-ketane-to-mohatla,ho itšetlehile ka sebaka sa lihlopha tse sebetsang ka har'a tatellano ea peptide.
Hlooho ho Mohatla
·E etsahala pakeng tsa N-terminus ho C-terminus.
·Ka Amide bond sebopeho (lactamization).
•Baesekele e sebelisoang khafetsa e fanang ka botsitso bo boholo.
Side Chain to Head
E etsahala lipakeng tsa N-terminus le COOH ea kahare
(mohlala. sehlopha sa ß-COOH sa Asp kapa γ-COOH-sehlopha sa Glu).
Lehlakoreng la Chain ho isa mohatleng
E etsahala lipakeng tsa NH2s le C-terminus
(mohlala. sehlopha sa ε-NH2-sehlopha sa Lys).
Ketane ea Mahlakoreng ho ea ho Ketane ea Mahlakoreng
E etsahala pakeng tsa Side chain of Lysine (ε-NH2–sehlopha sa Lys) ho aspartic kapa glutamic acid (γ-COOH-sehlopha).
Litlhaloso tse hlahang
Moralo le Lithuto tsa NMR tsa Cyclic Peptides Tse Lebisang N-terminal Domain ea Protein Tyrosine Phosphatase YopH
Re tlaleha ka moralo le tlhahlobo ea li-peptide tse ncha tse shebileng sebaka sa N-terminal sa protheine tyrosine phosphatase YopH ho tsoa Yersinia.. Li-peptide tsa cyclic li entsoe ho ipapisitsoe le tatellano e khutšoane ho tsoa ho protheine ea SKAP-HOM [TSA(pY)DDPF (pY = phosphotyrosine)], 'me kaofela li na le motif DEZXDPfK (moo Z e leng phosphotyrosine kapa e seng hydrolyzable phosphotyrosine mimetic, X ke asiti ea aspartic kapa leucine mme f ke d-phenylalanine). Li-peptide tsena li hlahisa moralo oa 'hlooho ho isa mohatleng', ho nolofalletsa cyclization ka ho theha maqhama a amide lipakeng tsa liketane tse ka thoko tsa asiti ea pele ea aspartic le masala a lysine.. Lithuto tsa phetoho ea lik'hemik'hale li entsoe ho lekola ho tlama ha li-peptide tsena sebakeng sa N-terminal sa YopH.. Li-peptide tse nang le karolo ea phosphotyrosine li bonts'a likamano tse tlamang sebakeng se tlase sa micromolar; Ho nkela sebaka sa phosphotyrosine le e 'ngoe ea lintho tse tsoang ho eona tse se nang hydrolyzable ho fokotsa haholo likamano tse tlamang.. Lithuto tsena tsa pele li ka bula tsela ea ho sibolloa ha li-ligands tse matla le tse khethang tsa sebaka sa YopH N-terminal tse ka batlisisoang ka matla a tsona a ho thibela ts'oaetso ea Yersiniae..
Litšebeletso tse Amanang tsa Phetoho ea Peptide
glycosylation ea peptide
glycosylation ea peptide
Peptide glycosylation ke phetoho e kopanetsoeng e ka ntlafatsang thepa ea physicochemical ea peptide.
Bala haholoanyanePhosphorylation ea peptide
Phosphorylation ea peptide
Phosphoralation e ka hlaha ka Serine (S, Ho ba), Threonine (T, Thr) le Tyrosine (Y, Tyr) liketane tse mahlakoreng ka ho theha bond ea phosphoester
Bala haholoanyaneLi-peptide tse tloaelehileng
Li-peptide tse tloaelehileng
Kenyelletso ea li-amino acid tse peli tseo e seng tsa tlhaho tse nang le α-methyl, lihlopha tsa α-alkenyl nakong ea motsoako o tiileng oa liketane tsa peptide.
Bala haholoanyanePeptide ea cyclic
Peptide ea cyclic
Peptide cyclization e ntlafatsa botsitso ba li-peptide (e amanang le li-analogue tsa bona tsa mela) 'me ke leano le tloaelehileng ho nts'etsopele ea peptide.
Bala haholoanyanephetoho ea peptide
Boiphihlelo bo pharalletseng ba phetoho ea peptide, ho fana ka mekhoa e mengata e sebetsang ea lipatlisiso tsa peptide.
