Gyaran Peptide
Isotope Labeled Peptides
Menene Isotope Labeled Peptides?
Stable isotope labeling wani sinadari reagent ne wanda a cikinsa kwayoyin na wani fili sun ƙunshi daya ko fiye da tsayayye atom na isotope kuma ana tantance su da ƙaddara ta amfani da keɓaɓɓen kaddarorin su..
Stable isotope labeling dabaru sun dogara ne akan ka'idar yiwa mai amsawa ta hanyar maye gurbin takamaiman atom tare da isotope., wanda sai ya sa mai amsawa ya sami amsa kuma ya gano atom tare da canjin lambar neutron, wanda za a iya sa ido ta hanyar halayen, hanyoyin metabolism ko sel. Isotope mai ƙarfi (13C, 15N, 13C15N) Ana amfani da peptides masu lakabi da yawa azaman 'alamomin sinadarai' a cikin ƙididdigar ƙididdiga don ganowa da gano samfuran halitta.. Hanyoyi masu ƙididdigewa suna amfani da ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun taro don raba alamar isotope da ions peptide mara lakabi ko peptide ions., kuma suyi amfani da ƙimar siginar da aka gano don ƙididdige ƙididdiga.
Fasahar alamar isotope mai tsayayye tana da fa'idodin babban hankali, matsayi mai sauƙi da daidaitaccen ƙididdigewa, wanda ke sa gyare-gyaren isotope ke samun kulawa sosai a fagen magani da biochemistry.
Isotope Labeled Peptides
Daidaitaccen Kayan Aikin Bincike
Stable isotope mai lakabin peptides ana haɗa su ta hanyar maye gurbin takamaiman atom a cikin amino acid na halitta tare da isotopes masu nauyi., kamar maye gurbin 12C da 13C, 14N da 15N, ko 1H da 2H (deuterium). Waɗannan gyare-gyaren suna haifar da mahaɗan masu lakabi na isotope waɗanda ke riƙe da kaddarorin physiochemical iri ɗaya da amsawar sinadarai kamar takwarorinsu marasa labeled. Wannan ya sa su zama makawa a cikin ɗimbin aikace-aikacen kimiyya, ciki har da mass spectrometry, Nazarin NMR, da kuma nazarin pharmacokinetic.
|
Amino acid
|
Isotope
|
Bambancin taro zuwa AA mara lakabi
|
|---|---|---|
|
Alanine |
U- 13C3, U-15N |
+4 Da |
|
Arginine |
U- 13C6, U-15N4 |
+10 Da |
|
Leucine |
U- 13C6, U-15N |
+7 Da |
|
Lysine |
U- 13C6, U-15N2 |
+8 Da |
|
Phenylalanine |
U- 13C9, U-15N |
+10 Da |
|
Proline |
U- 13C5, U-15N |
+6 Da |
|
Valine |
U- 13C5, U-15N |
+6 Da |
Fitattun Bayani
Yin amfani da alamar isotope tsayayye don haɓaka fahimtarmu game da ilimin etiology da cututtukan cututtukan Alzheimer
Tsayayyen alamar isotope tare da yawan gani (MS)-tushen bincike na proteomic ya zama dabara mai ƙarfi don tantance matakan tsayayyen furotin, juyayin furotin, da kuma gurɓataccen furotin. Yin amfani da waɗannan dandamali na nazarci zuwa cututtukan neurodegenerative na iya buɗe sabbin abubuwa na etiology na waɗannan cututtuka masu lalacewa.. Kwanan nan, Bargarin isotopes-MS an yi amfani dashi don bincika hanyoyin cututtukan cututtukan farko na cutar Alzheimer (AD) da linzamin kwamfuta model na AD-kamar Pathology. A cikin wannan bita, mun taƙaita waɗannan tsayayyen ƙirar gwajin isotope-MS da aikace-aikacen kwanan nan a cikin mahallin AD Pathology. Mun kuma bayyana ƙoƙarinmu na yanzu da nufin yin amfani da ƙarfin maganadisu na nukiliya (NMR) nazarin barga isotope mai lakabi amyloid fibrils daga AD linzamin kwamfuta model kwakwalwa. Gaba ɗaya, waɗannan hanyoyin suna ba da sabbin damar yin nazarin sauye-sauyen proteome a cikin AD da sauran cututtukan neurodegenerative ta hanyar haɓaka hanyoyin da za a yi niyya don jiyya da rigakafin..
Abubuwan Gyaran Peptide masu alaƙa
peptide glycosylation
peptide glycosylation
Peptide glycosylation shine gyare-gyare na covalent wanda zai iya inganta halayen physicochemical na peptides.
Kara karantawapeptide phosphorylation
peptide phosphorylation
Phosphoralation na iya faruwa akan Serine (S, Don zama), Threonine (T, Thr) da kuma Tyrosine (Y, Tyr) sarƙoƙi na gefe ta hanyar haɗin phosphoester
Kara karantawaPeptides masu tasowa
Peptides masu tasowa
Gabatarwar amino acid guda biyu marasa dabi'a wadanda ke dauke da α-methyl, Ƙungiyoyin α-alkenyl yayin haɓakaccen lokaci mai ƙarfi na sarƙoƙi na peptide.
Kara karantawaCyclic Peptide
Cyclic Peptide
Juyin hawan peptide yana haɓaka kwanciyar hankali na peptides (dangane da analogues na layin su) kuma dabara ce ta gama gari a cikin ci gaban peptide.
Kara karantawapeptide gyara
Ƙwarewa mai zurfi a cikin gyaran peptide, samar da hanyoyi masu dacewa da yawa don binciken peptide.
