Gyaran Peptide
Gwajin Peptide
Matsayin gwajin peptide
Gwajin Peptide yana ƙayyade tsabta, nauyin kwayoyin halitta, daidaitattun jerin, da kasancewar rashin tsabta na peptides. Wannan yana da mahimmanci ga gwaje-gwajen binciken kimiyya, ci gaban miyagun ƙwayoyi da aikace-aikacen asibiti saboda: high tsarki peptides (misali. >95%) zai iya rage tsangwama na gwaji da inganta amincin bayanai. Rashin tsabta peptides na iya shafar ayyukan nazarin halittu ko ma haifar da amsawar rigakafi (misali. ƙazanta a cikin ƙwayoyi na iya haifar da alerji ko guba).
Gwaje-gwajen da aka saba amfani da su:
HPLC (Chromatography Liquid Babban Ayyuka): yana nazarin tsaftar peptide da ƙazanta abun da ke ciki.
Mass Spectrometry (MS): Ƙayyade nauyin kwayoyin kuma tabbatar da daidaiton jeri.
Amino acid bincike (AAA): ƙididdige adadin amino acid ɗin peptide.
Tabbatar da ayyukan nazarin halittu da aikin peptides
Tabbatar da kwanciyar hankali na peptide reagents
Tabbatar da haihuwa da kuma yarda da endotoxin
Gwajin Peptide gama gari
Ya fi haɗa da tsabtar peptides; gwajin abun ciki; gwajin haifuwa; PH darajar; gwajin danshi; gwajin solubility da sauran fannoni.
Haihuwa
Hanyar allurar kai tsaye: allura a cikin kafofin watsa labarai biyu, shirya don 14 kwanaki da kuma kiyaye ga microbial girma.
Hanyar tacewa membrane: Maganin da aka wuce ta bakararre tace membrane (girman pore 0.22 μm), microorganisms sun kasance tarko a kan membrane, wanda aka canjawa wuri zuwa cibiyar al'adu don shiryawa.
ATP bioluminescence: Gano ATP da aka samar ta hanyar ƙwayoyin cuta, m (hours) amma yana buƙatar tabbatarwa.
Tsafta
Farashin RP-HPLC: Dangane da bambancin hydrophobicity na peptides, rabuwa akan ginshiƙin C18/C8 da ganowa ta UV ko MS.
MS: Ƙaddamar da nauyin kwayoyin peptide (MALDI-TOF ko ESI-MS), tabbatar da yarjejeniya tare da ƙididdiga na ƙididdiga da gano gyare-gyare ko ƙazanta.
Amino acid tsarin bincike: Bayan acid hydrolysis na peptide, ƙididdige abubuwan da ke tattare da kowane amino acid kuma tabbatar da tsabta ta kwatanta da ƙimar ka'idar.
SDS-SHAFIN: Rabuwa ta hanyar electrophoresis da lura da makada guda ɗaya ta Caulmers Brilliant Blue ko Tabon Azurfa.
abun ciki
Binciken abubuwa: Ta hanyar ɓarna hadaddun tsarin jiki zuwa ƴan kankanin “tsari-tsari” da yawa., kowane “tsari-tsari” yana bin doka ɗaya, Ana sauƙaƙa matsalar ta hanyar nazarin waɗannan “tsari-tsari” da amfani da hanyoyin ilimin lissafi..
Kiyaye taro: A kowane hali na jiki ko sinadarai, jimlar yawan abubuwan da ke cikin abin da ke cikin abin da ya faru daidai yake da jimlar samfuran bayan amsawa.
Daidaita yanki:Wannan hanya ce da aka ayyana ta hanyar lissafi azaman mai da cikakkiyar ƙimar tsarin jiki zuwa alaƙar ƙima ta dangi.. Ta hanyar daidaitawa, ana iya sauƙaƙe lissafin kuma ana iya rage ƙimar yadda ya kamata.
Gwajin Peptide
Sami kwatancen gwajin peptide don bin diddigin yuwuwar aikin gwajin ku.
