Fmoc-hArg(Pbf)-OH wani ci-gaba na amino acid wanda bai dace ba sanye yake da ƙungiyoyin kariyar orthogonal, musamman ingantacce don daidaitaccen tsarin Fmoc m-lokaci peptide kira (SPSS). Ta hanyar gabatar da homoarginine (h A fusace)-wanda ya ƙunshi ƙarin ƙungiyar methylene fiye da arginine na halitta-a cikin jerin peptide, yana ƙara haɓaka kwanciyar hankali na proteolytic da ingantaccen shigar da ƙwayoyin peptide na salula. Sarkar gefen sa yana da kariya ta ƙungiyar Pbf-ma'auni na masana'antu, wanda ke da labile sosai a lokacin trifluoroacetic acid (TFA) tsaga, yadda ya kamata rage girman halayen gefe. Yana da kyakkyawan tubalin gini don haɗa tsafta mai ƙarfi, hadaddun gyara peptides.
Jeri
Fmoc-hArg(Pbf)-OH
Lambar CAS
1159680-21-3
Tsarin kwayoyin halitta
Saukewa: C35H42N4O7S
Nauyin Kwayoyin Halitta
662.80
Chemical & Abubuwan Amfanin Magana Fmoc-hArg(Pbf)-OH yayi daidai da zamani Fmoc/tBu m-lokaci peptide kira dabarun. A cikin kira peptide, Kariya da kariyar ƙungiyar guanidino mai ƙarfi babban ƙalubale ne. Sashin gefen wannan samfurin yana amfani da Pbf (2,2,4,6,7-pentamethyldihydrobenzofuran-5-sulfonyl) kungiyar karewa. Idan aka kwatanta da na gargajiya Pmc ko Mtr kungiyoyin, Pbf yana ba da fa'idodi masu yawa:
- Rage Ragewa: A lokacin matakin tsagewar TFA na ƙarshe, Yawan cire Pbf yana da sauri da sauri fiye da sauran ƙungiyoyin kare sulfonyl.
- Danne Ra'ayin Side: Saurin karewa da kyau yana rage haɗarin halayen halayen alkylation wanda ke haifar da carbocation akan sassan sassan amino acid masu mahimmanci kamar tryptophan. (Trp), ta haka ne sosai inganta tsafta da yawan amfanin danyen peptide.
Aikace-aikacen Frontier a cikin Gano Magungunan Peptide A matsayin amino acid mara kyau, homoarginine (h A fusace) yana da kwarangwal na carbon wanda shine methylene daya ya fi tsayi fiye da arginine na halitta. A cikin sabon ƙirar ƙwayoyi, wannan ƙananan gyare-gyaren tsarin yana haifar da fa'idodi masu mahimmanci na nazarin halittu:
- Tsarin Enzymatic: Proteases na halitta (irin su Trypsin) yawanci gane da kuma raba C-terminus na Arg ko Lys musamman. Maye gurbin Arg tare da hArg mara kyau yana ba da damar peptide don gujewa ganewar protease yadda ya kamata., Ta haka yana haɓaka rabin rayuwar magungunan peptide a cikin jini sosai.
- Ingantaccen Ciwon Hannun Hannu: A cikin binciken da ya ƙunshi peptides masu shiga cikin tantanin halitta mai arzikin guanidino (CPPs), ƙarin sassaucin sararin samaniya da hArg ke bayarwa yana haɓaka yanayin ɗaure tsakanin ƙungiyar guanidino da heparan sulfate akan farfajiyar tantanin halitta., don haka haɓaka haɓaka haɓakar isar da isar da isar da isar da isar da isar da magunguna na macromolecular.
COA
HPLC
MS
- Haɓaka Farko da Fa'idodin Sinadarai na Ƙungiyar Kare Pbf:
Hornbeam, L. A., Shroff, H., Triol, S. A., Mansur, E. M., Wenschuh, H., & Albericio, F. (1993). “Ƙungiyar 2,2,4,6,7-pentamethyldihydrobenzofuran-5-sulfonyl (Pbf) as arginine gefen sarkar kariya.” Jaridar American Chemical Society, 115(10), 4397-4398. Tushen Tabbatarwa: DOI: 10.1021/ku 00063a082
- Daidaitaccen Ka'idoji don Fmoc SPPS da Aikace-aikacen Pbf:
Chan, W. C., & Fari, P. D. (2000). Fmoc Solid Phase Peptide Synthesis: Hanya Mai Aiki. Jami'ar Oxford Press. (Duba shafuka 58-60 don daidaitattun aikace-aikace na kariyar sarkar gefe-sarkar arginine). Tushen Tabbatarwa: ISBN: 978-0199637249
- Ƙimar Halitta na Tsawon Sarkar Side (Homoarginine) akan Haɓaka Samuwar Hannu:
Wender, P. A., Mitchell, D. J., Pattabiraman, K., Pelkey, E. T., Steinman, L., & Rothbard, J. B. (2000). “Zane, kira, da kuma kimanta ƙwayoyin da ke taimakawa ko haɓaka ɗaukar salula: Masu jigilar kwayoyin halittar peptoid.” Abubuwan da aka gabatar na Kwalejin Kimiyya ta Kasa, 97(24), 13003-13008. Tushen Tabbatarwa: DOI: 10.1073/shafi.97.24.13003
