C-Terminal Peptide gyare-gyare Yana Bayyana Matsayin Kai tsaye da Kai tsaye na Cholesteroylation na Bushiya Morphogen

Bushiya (Hh) morphogens suna da hannu a cikin haɓakar amfrayo da ilimin halitta mai tushe da kuma, idan aka yi kuskure, zai iya ba da gudummawa ga ciwon daji. Ɗaya daga cikin mahimman gyare-gyare bayan fassarar fassarar tare da tasiri mai zurfi akan aikin Hh shine cholesteroylation C-terminal yayin biosynthesis.. Hasashen na yanzu shine cewa ƙwayar cholesterol wani muhimmin al'amari ne a cikin haɗin gwiwar Hh tare da takardan membrane na plasma na waje na samar da sel., cell-surface Hh multimerization, da sufuri da sigina. Duk da haka, ba a yanke shawarar ko ƙwayar cholesterol tana da hannu kai tsaye a cikin duk waɗannan hanyoyin ba, saboda haɗin gwiwar aikin su yana haifar da madadin yiwuwar cewa cholesterol ya fara aiwatarwa da wuri kai tsaye kuma waɗannan hanyoyin za su iya tafiyar da matakai na gaba na siginar Hh ba tare da lipid ba.. Mun ƙirƙira bambance-bambancen C-terminal Hh peptide kuma mun lura cewa waɗannan peptides na cholesteroylated sun bambanta da yawa hanyoyin fassarar bayan fassarori a cikin samar da sel da Hh biofunction a cikin Drosophila melanogaster ido da haɓaka reshe.. Mun kuma gano cewa adadin Hh masu yawa da aka ware daga alamun peptide na cholesteroylated a cikin vitro da in vivo da kuma waɗanda aka yiwa alama da bambance-bambancen Hh waɗanda ba su da abubuwan C-cholesterol ɗin su sun kasance masu ƙarfi.. Hanyarmu ta haka ta tabbatar da cewa Hh cholesteroylation yana da mahimmanci yayin farkon matakan samar da Hh da maturation amma kuma yana nuna cewa ana iya raba shi don karɓar siginar Hh a karɓar sel..