Huringa Peptide
peptide Phosphorylation
He aha te peptide Phosphorylation?
Ko te phosphorylation pūtau he whakarerekētanga ka taea te huri i te mahi, āhuatanga here, ko te waahi o te pūtau o te pūmua me te whākōkī. He mahinga ture nui ano hoki e whai waahi nui ana ki te maha o nga pathologies mate, tae atu ki te mumura, mate pukupuku, mate neurological me te metabolic. Ko nga Enzymes e phosphorylate pūmua ki nga toenga waikawa amino motuhake (e.g., tirohina, serine, me te toronine) ka kiia he kinase. Ko nga enzymes e tango ana i te roopu phosphate ma te hydrolysis ka kiia ko te phosphatases. Na te mea ko te phosphorylation te pokapū o te nuinga o nga huarahi whakawhiti tohu, peptides phosphorylated (i.e., phosphopeptides) ka whakamahia mo te wetewete i nga kinases pūmua me te phosphatases i roto i nga whakamatautau pūkoro.
Te waahi o nga roopu phosphoryl
Ka puta pea te Phosphoralation ki te Serine (S, Kia noho), Threonine (T, Thr) me Tirohina (Y, Tiro) nga mekameka taha na te hanganga here phosphoester. Ka taea e CPC Scientific te whakakotahi i nga phosphopeptides me te whakauru i nga huinga kotahi, maha ranei o te phosphoserine (pS), phosphothreonine (pT), phosphotyrosine ranei (pY).
Phosphoserine
Ko te Phosphoserine he peptide poto, he mekameka peptide ranei kei roto he serine phosphorylated (pSer), i.e., he kongakonga peptide kei roto te roopu hydroxyl (-OH) o te toenga serine ka whakarereketia e te roopu phosphate (-PO₄²-).
Phosphothreonine
Ko te Phosphothreonine he peptide poto, he mekameka polypeptide kei roto ko te phosphothreonine (pThr), i.e. he peptide kei roto te roopu hydroxyl (-OH) o te toenga threonine ka whakarereketia e te roopu phosphate (-PO₄²-).
Phosphotyrosine
Ko te Phosphotyrosine he peptide poto, he mekameka polypeptide kei roto te phosphotyrosine (pTyr), i.e. he peptide kei roto te roopu hydroxyl phenolic (-OH) o te tyrosine (Tiro) Ko te toenga ka whakarereketia e te roopu phosphate (-PO₄²-).
Nga Tirohanga Whakaaturanga
Ko te PRMT5 C-terminal Phosphorylation e Whakaritea ana he 14-3-3/PDZ Whakawhiti Whakawhitiwhiti*
Ko te PRMT5 te whākōkī tuatahi mō te tukunga o te dimethylarginine hangarite i roto i ngā pūtau whakangote. I roto i te kaha ki te mohio me pehea te whakahaere a PRMT5, I tautuhia e matou he waahi phosphorylation threonine i roto i te kaupapa hiku C-terminal, e arohia ana e te Akt / serum- me nga kinases glucocorticoid-inducible. I a koe e whakatewhatewha ana i te mahi o tenei whakarereketanga i muri i te whakamaoritanga, ka kitea e matou ko tana hiku C-terminal kore utu e herea ana nga rohe PDZ (ka unphosphorylated) a 14-3-3 pūmua (ka phosphorylated). Ko te tikanga, he takahanga phosphorylation i roto i nga toenga whakamutunga o te hiku C-terminal ka whakaputa i te "whakawhiti" PDZ/14-3-3 e whakawhirinaki ana ki te whakarereketanga. Ko te kaupapa C-terminal o PRMT5 e hiahiatia ana mo te hononga membrane plasma, me te ngaronga o tenei kaha whakawhiti kaore i te hototahi ki te oranga. I purongohia tenei ahuatanga tohu mo te HPV E6 oncoprotein engari kaore ano kia kitea mo nga pūmua whakangote.. Ki te tirotiro i te kaha o te whakawhiti PDZ/14-3-3 i te whakawhiti tohu, hanga matou he microarray rohe pūmua e whanga ana rohe PDZ me 14-3-3 pūmua. I whakamahia e matou tenei microarray ki te uiui i nga hiku C-terminal o te roopu iti o nga pūmua kaitono me te tautuhi i te ERBB4, PGHS2, me te IRK1 (me te E6 me te PRMT5) kia rite ki tenei aratau tohu, ko te whakaaro ko te whakawhiti PDZ/14-3-3 he tauira koiora whanui.
Ratonga Whakarerekē Peptide e Pa ana
peptide glycosylation
peptide glycosylation
Ko te peptide glycosylation he whakarerekētanga kawau ka taea te whakapai ake i nga ahuatanga matū-ahua o nga peptides.
Pānuitia atupeptide Phosphorylation
peptide Phosphorylation
Ka puta pea te Phosphoralation ki te Serine (S, Kia noho), Threonine (T, Thr) me Tirohina (Y, Tiro) nga mekameka taha na te hanganga here phosphoester
Pānuitia atuPeptides Stapled
Peptides Stapled
Te whakaurunga o nga waikawa amino e rua, he α-methyl, Ko nga roopu α-alkenyl i te wa e whakahiatotia ana te wahanga totoka o nga mekameka peptide.
Pānuitia atuPeptide Porohita
Peptide Porohita
Ko te paheketanga o te peptide e whakanui ana i te whakapumautanga o nga peptides (e pa ana ki o raatau analogues raina) a he rautaki noa mo te whanaketanga peptide.
Pānuitia atuwhakarerekētanga peptide
He wheako nui ki te whakarereke peptide, te whakarato i nga huarahi maha mo te rangahau peptide.
