LL-37 da antimicrobial peptide daga cathelicidin iyali. Sunan kansa yana nufin sa 37 amino acid abun da ke ciki da kuma N-terminal farawa na biyu leucine saura. HCAP18, kadai memba na mutum cathelicidin iyali, yana jurewa takamaiman ɓarna ta proteases na ɗan adam don sakin wannan guntun LL-37 mai aiki da ilimin halitta..
Gano LL-37 ya nuna alamar canjin bincike na cathelicidin daga ƙirar dabba zuwa ga mutane. A matsayin muhimmin sashi na tsarin rigakafi na asali, LL-37 yana taka muhimmiyar rawa wajen kare ɗan adam daga ƙwayoyin cuta.
Babban aikin wannan tsaro shine babban aikin kashe kwayoyin cuta kai tsaye. Amphipathic α-helical(Alpha helix) Tsarin da ke da alhakin wannan aikin yana haifar da jan hankali na electrostatic zuwa ga membranes na kwayan cuta mara kyau. Rushewar mutuncin membrane kai tsaye yana haifar da asarar abubuwan gina jiki na cikin salula; cikakken mutuwar kwayoyin cuta ya biyo baya. Musamman, wannan tsarin ba ya haifar da kowane nau'i na juriya.
Sauran ayyuka a cikin jiki kuma ana danganta su da shi – warkar da rauni, gyaran nama, m immunomodulation, endotoxin neutralization, da kuma nuna anti-cancer da anti-viral effects.
Jeri
Leu-Leu-Gly-Asp-Phe-Phe-Arg-Lys-Ser-Lys-Glu-Lys-Ile-Gly-Lys-Glu-Phe-Lys-Arg-Ile-Val-Gln-Arg-Ile-Lys-Asp-Phe-Leu-Arg-Glu-AV-Asn
Lambar CAS
154947-66-7
Tsarin kwayoyin halitta
Saukewa: C205H340N60O53
Nauyin Kwayoyin Halitta
4493.34
LL-37 Bincike mai alaƙa
1.Broad-Spectrum Kai tsaye Ayyukan Kwayoyin cuta
Rapid da kai tsaye bactericidal mataki a kan m iri-iri microorganisms is LL-37’s most well documented function. Gram negative, positive, and even some fungal pathogens are all effectively killed.
The cationic, amphipathic characteristics of the peptide are key to this process; charged segments bind directly to bacterial membranes. Leakage of cellular contents and collapse of membrane potential are the end results of the attack.
Traditional antibiotics work through very different (and often target-specific) resistance pathways; LL-37’s mode of attack is both fast and, to date, not associated with significant resistance development. Human clinical studies support the safety and efficacy of LL-37 as a therapeutic agent.
2.Powerful Immunomodulatory Function
LL-37 exerts its effects based on the local microenvironment within the body. Upon detection of tissue injury or pathological changes, LL-37 yana kunna abubuwan tsari daban-daban da sel don tarawa a waɗannan rukunin yanar gizon.
Daidaitawar sakin cytokine mai kumburi yana ɗaya daga cikin hanyoyinsa na farko; wuce kima pro-mai kumburi martani aka musamman downregulated. Inganta dendritic cell(DC) maturation da kuma na gaba T cell bambancewa wani muhimmin al'amari ne na wannan tsari – wakiltar wata muhimmiyar gada tsakanin rigakafi na asali da kuma daidaitawa.
3.Haɓaka Warkar da Rauni da Gyaran Nama
LL-37's raunin warkarwa da ikon gyara nama suna da alaƙa kai tsaye zuwa ayyukan immunomodulatory, tare da aikin antimicrobial kai tsaye.
Kunna hanyoyin antimicrobial yana haifar da keratinocytes, fibroblasts, da kuma ƙwayoyin endothelial don yin ƙaura zuwa wurin rauni; yaduwar kwayar halitta yana biyo bayan lalacewa yayin da aka gyara lalacewa. Angiogenesis yana ba da duka makamashi da isar da abinci mai gina jiki zuwa nama mai rauni.
4.Ayyukan rigakafin ciwon daji
Kwayoyin ciwon daji gabaɗaya suna raba halayen gama gari na fallasa ƙarin caji mara kyau phosphatidylserine a jikin jikinsu na waje (Kwayoyin al'ada suna da nasu gida na waɗannan phospholipids). Kyakkyawan cajin LL-37 don haka yana jan hankalin su.
Apoptosis da necrotic hanyoyin mutuwa masu kama da waɗanda aka yi amfani da su akan ƙwayoyin cuta sannan an fara. Kai tsaye anti-tumor Tasiri ta hanyar daidaita yanayin rigakafi shima yana taka rawa.
Nazarin preclinical ya nuna raguwar raguwar ƙwayoyin cutar kansa da aka bi da su tare da LL-37; maganin ciwon daji ta amfani da LL-37 yanki ne mai ban sha'awa na bincike.
COA
HPLC
MS
