Gịnị bụ N-terminal mgbanwe?

Ọbá akwụkwọ peptide sitere na proteome maka nkọwapụta miri emi nke reagents mgbanwe N-terminal

Protein na peptide N termini bụ ebumnuche dị mkpa maka mgbanwe mgbanwe na chemoproteomics reagents na ngwaọrụ bioconjugation.. N-terminal α-amine na-apụta naanị otu ugboro na yinye polypeptide ọ bụla, na-eme ka ọ bụrụ ebumnuche mara mma maka bioconjugation protein. N'ime sel, Enwere ike iwepụta njedebe N ọhụrụ site na mgbawa proteolytic wee weghara ya site na N-terminal modification reagents nke na-eme ka njirimara proteome n'obosara nke ihe protease na tandem mass spectrometry. (LC-MS/MS). Nghọta nke usoro N-terminal kpọmkwem nke reagents mgbanwe dị oke mkpa maka ngwa ndị a ọ bụla.. Ọbá akwụkwọ peptide sitere na Proteome yana LC-MS/MS bụ ngwaọrụ siri ike maka ịkọwapụta usoro nkọwapụta nke N-terminal modification reagents.. Ụlọ akwụkwọ ndị a dị nnọọ iche iche, na LC-MS/MS na-enyere aka nyochaa arụmọrụ mgbanwe nke iri puku kwuru iri puku usoro na otu nnwale.. Ọbá akwụkwọ peptide sitere na proteome bụ ngwá ọrụ siri ike maka ịkọwapụta usoro nkọwapụta nke enzymatic na peptide peptide labeling reagents.. Subtiligase na 2-pyridinecarboxaldehyde (2PCA) bụ reagents abụọ emepụtara maka mgbanwe N-terminal peptide nhọrọ ma enwere ike mụọ ya site na iji ọba akwụkwọ peptide enwetara proteome.. Usoro a na-akọwapụta usoro maka ịmepụta ọba akwụkwọ peptide sitere na N-n'ikpeazụ dị iche iche yana maka itinye ụlọ akwụkwọ ndị a na profaịlụ nkọwa nke N-terminal modification reagents.. Anyị na-akọwa usoro maka ịkọwapụta nkọwapụta nke 2-pyridinecarboxaldehyde, a chemical mgbanwe reagent, na subtiligase, enzymatic mgbanwe reagent. Enwere ike ịmegharị usoro iwu ndị a n'ụzọ dị mfe na isi mmalite proteome ọzọ yana ndị ọzọ N-terminal peptide labeling reagents..