Fmoc-hArg(Pbf)-OH bụ ihe mgbapụta amino acid na-ekwekọghị n'okike nke nwere otu nchekwa orthogonal, kachasị maka ọkọlọtọ Fmoc solid-phase peptide njikọ (SPSS). Site na iwebata homoarginine (h Iwe)-nke nwere otu methylene ọzọ karịa arginine eke - n'ime usoro peptide, ọ na-akwalite nkwụsi ike nke proteolytic na ntinye ntinye nke cellular nke ọgwụ peptide. Otu Pbf ọkọlọtọ ụlọ ọrụ na-echekwa yinye akụkụ ya, Nke a na-eme nke ọma n'oge trifluoroacetic acid (TFA) ikpochapu, na-ebelata mmeghachi omume akụkụ nke ọma. Ọ bụ ihe mgbochi ụlọ dị mma maka ịmepụta ịdị ọcha dị elu, mgbagwoju gbanwetụrụ peptides.
Usoro
Fmoc-hArg(Pbf)-OH
Nọmba CAS
1159680-21-3
Usoro ihe omimi
C35H42N4O7S
Ibu molekụla
662.80
Chemical & Uru Synthesis Fmoc-hArg(Pbf)-OH dabara nke ọma na atụmatụ Fmoc/tBu siri ike-fase peptide njikọ nke ọgbara ọhụrụ. Na njikọ nke peptide, Nchedo na nchebe nke otu guanidino siri ike bụ isi ihe ịma aka. Agbụ akụkụ nke ngwaahịa a na-eji Pbf (2,2,4,6,7-Pentamethyldihydrobenzofuran-5-sulfonyl) otu nchekwa. Tụnyere otu Pmc ma ọ bụ Mtr ọdịnala, Pbf na-enye uru dị ukwuu:
- Nhicha ngwa ngwa: N'oge ikpeazụ TFA cleavage nzọụkwụ, Mwepụ nke Pbf dị ngwa ngwa karịa otu ndị ọzọ na-echebe sulfonyl.
- Mbelata mmeghachi omume akụkụ: Mwepu ngwa ngwa na-ebelata ihe ize ndụ nke mmeghachi omume akụkụ alkylation nke carbocation na-akpata n'agbụ akụkụ nke amino acid dị nro dị ka tryptophan. (Tp), si otú ahụ na-emeziwanye ịdị ọcha na mkpụrụ nke peptide crude.
Ngwa Frontier na nchọpụta ọgwụ Peptide Dị ka amino acid na-ekwekọghị n'okike, homoarginine (h Iwe) nwere skeleton carbon nke bụ otu methylene ogologo karịa arginine eke. Na nhazi ọgwụ ọhụrụ, Ngbanwe nhazi a dị ntakịrị na-ewepụta uru dị ukwuu nke ndu:
- Enzymatic kwụsie ike: Eke proteases (dị ka trypsin) na-amata ma kewaa C-terminus nke Arg ma ọ bụ Lys kpọmkwem. Dochie Arg na hArg na-ekwekọghị n'okike na-enye ohere ka peptide gbanahụ nnabata protease nke ọma., si otú ahụ gbatịkwuo ọkara ndụ ọgwụ peptide na plasma.
- Mbulite ekwentị emelitere: Na nyocha metụtara guanidino-ọgaranya peptides na-abanye cell (CPP), Mgbanwe ohere ọzọ nke hArg na-enye na-eme ka ọnọdụ njikọ dị n'etiti otu guanidino na heparan sulfate n'elu akpụkpọ ahụ cell., si otú a na-emeziwanye arụmọrụ nnyefe transmembrane nke ọgwụ macromolecular.
COA
HPLC
MS
- Mmepe izizi na uru kemịkalụ nke Otu Nchekwa Pbf:
Hornbeam, L. A., Shroff, H., Triol, S. A., Mansour, E. M., Wenschuh, H., & Albericio, F. (1993). “Otu 2,2,4,6,7-pentamethyldihydrobenzofuran-5-sulfonyl (Pbf) dị ka arginine n'akụkụ yinye nchebe.” Akwụkwọ akụkọ nke American Chemical Society, 115(10), 4397-4398. Isi mmalite nkwenye: DOI: 10.1021/ja00063a082
- Ụkpụrụ ọkọlọtọ maka Fmoc SPPS na ngwa Pbf:
Chan, W. C., & Ọcha, P. D. (2000). Fmoc Solid Phase Peptide Synthesis: Ụzọ Kwesịrị Ekwesị. Mahadum Oxford Press. (Lee ibe 58-60 maka ngwa ọkọlọtọ nke nchebe akụkụ nke arginine). Isi mmalite nkwenye: ISBN: 978-0199637249
- Nkwado ndu nke Ogologo akụkụ-Chain (Homoarginine) na ịkwalite nkwalite cellular:
Wender, P. A., Mitchell, D. J., Pattabiraman, K., Pelkey, E. T., Steinman, L., & Rothbard, J. B. (2000). “Nhazi, njikọ, na nleba anya nke ụmụ irighiri ihe na-enyere ma ọ bụ kwalite iweli ekwentị: Ndị na-ebugharị molecular peptoid.” Usoro nke National Academy of Sciences, 97(24), 13003-13008. Isi mmalite nkwenye: DOI: 10.1073/pnas.97.24.13003
