Kenya Sehlooho sa Hao Mona
Fluorescent Dye-Labeled Peptides
Tlhahiso ea rona
Peptide e ka ngoloa ka a dae e le 'ngoe kapa e mengata(s) ka peptide N-terminus, C-terminal, kapa ka hare, bakeng sa lisebelisoa tsa protheine lithuto tsa tšebelisano, ho lekola boteng ba li-biomolecules ho lisele, liteko tsa ts'ebetso ea enzyme, etc.
Bakeng sa li-peptide tse ngotsoeng ka dae le quencher, bona tsa rona LI-peptide tse TSOANG leqephe.
feie: Ho amoheloa ha CPP ka lisele tsa HeLa. Lisele tsa HeLa li ne li kentsoe ka OptiMEM medium e nang 10 uM FITC-LC-Antennapedia, katse # AS-24175, AS-24176 bakeng sa 1 h, e hlatsuoa le ho hlahlobjoa ka microscope ea fluorescence.
Lidae tsa rona tse nang le fluorescent li nka sebaka sohle se bonahalang le se haufi le lebone la infrared
HiLyte™ Fluor Dyes (pH ha e amehe)
HiLyte™ Fluor dyes are ideal for applications at high or low pH (11). These dyes also exhibit higher sensitivities, owing to their enhanced intensity and photostability.
| Lebitso | Abs/Em | Applications | |
|---|---|---|---|
| HiLyte™ Fluor 488 | • | 497/525 nm | FRET Assays, tracing or interaction studies |
| HiLyte™ Fluor 555 | • | 550/566 nm | TSOHLE, Ho ngola |
| HiLyte™ Fluor 647 | • | 650/675 nm | FRET/TR-FRET, Ho ngola |
| HiLyte™ Fluor 750 | • | 753/778 nm | Imaging |
Classical Dyes
Classical dyes such as FAM, TAMRA and the CyLyte Fluor dyes (Cy Dyes analogues) are a great cost-effective choice when operational pH range of your application is flexible.®
| Lebitso | Abs/Em | Applications | |
|---|---|---|---|
| Mca | • | 325/393 nm | TSOHLE |
| EDANS | • | 335/493 nm | TSOHLE |
| FAM | • | 492/518 nm | TSOHLE, Ho ngola |
| FITC | • | 494/519 nm | Ho ngola |
| CyLyte Fluor 3 | • | 550/564 nm | TSOHLE, Ho ngola |
| TAMRA | • | 541/568 nm | TSOHLE, Ho ngola |
| Rox | • | 568/591 nm | TSOHLE, Ho ngola |
| CyLyte Fluor 5 | • | 648/663 nm | FRET/TR-FRET, Ho ngola |
| CyLyte Fluor 7 | • | 750/773 nm | TSOHLE, Ho ngola |
Fluorogenic Substrates
Fluorogenic substrates do not require a quencher and contain a C-terminal dye that does not fluoresce until it is cleaved from the peptide (fluorescent form of dye is released).
| Lebitso | Abs/Em | Applications | |
|---|---|---|---|
| AMC | • | 351/430 nm | Fluorogenic substrates |
| AFC | • | 382/480 nm | Fluorogenic substrates |
| Rh110 | • | 501/527 nm | Fluorogenic substrates |
Litlhaloso tse hlahang
Peptide e Itšetlehileng ka pH le Mocheso E Tlamahanang ho Lactococcus lactis Oligopeptide-Binding Protein A E Lekoa ka Fluorescence Anisotropy Assay.
Stevie Norcross, Ashwin Sunderraj, Mathew Tatama
ACS Omega 2019, 4, 2812-2822, Hlakola 2006 2019 DOI: 10.1021/acsomega.8b02427
E tsoa ho AnaSpec: The sulforhodamine 101 e bitsoang bradykinin peptides (SR101-RPPGFSPFR le RPPGFSPFRK-SR101) li ile tsa etsoa 'me tsa hloekisoa ka HPLC (>90% bohloeki)
Redox Kinetics ea Amyloid-β-Cu Complex le Liphello Tsa Likokoana-hloko Tsa Tsona.
Paul Girvan, Xiangyu Teng, Nicholas J. Brooks, Geoffrey S. Baldwin, le Liming Ying
Biochemistry 2018, 57, 6228-6233 DOI : 10.1021/acs.biochem.8b00133
E tsoa ho AnaSpec: Amyloid-beta peptide e fetotsoeng ka fluorescent e nang le masalla a tšepe a tlamang ho ithuta ka litšobotsi tsa eona tsa tšepe le kinetics..
Ho arola le ho holisa Lisele tsa Endothelial Progenitor tse tsoang ho Peripheral Blood holim'a Peptide-functionalized Polystyrene Surfaces..
Mohamed A. Elkhodir, Mariève D. Boulanger, Omar Bashth, Jean-François Tanguay, Gaétan Laroche, Corinne A. Husli
Biotechnology le Bioengineering 2019, 116, 10, 2598-2609 DOI: 10.1002/bit.27107
E tsoa ho AnaSpec: Peptide e nang le RGD e nang le fluorophore, e bitsoang RGD-TAMRA e ile ea etsoa 'me ea hloekisoa ka HPLC (>90% bohloeki)
Lihlahisoa tse amanang & ditshebeletso
Li-peptide tsa TR-FRET
Lidae tsa Fluorescent
Re hlahisa li-peptide tse tloaelehileng tse ngotsoeng ka tšepe ea lanthanide e le mofani
Bala haholoanyaneLi-peptide tsa TR-FRET
Lidae tsa Fluorescent
Re hlahisa li-peptide tse tloaelehileng tse ngotsoeng ka tšepe ea lanthanide e le mofani
Bala haholoanyaneLi-peptide tsa TR-FRET
Lidae tsa Fluorescent
Re hlahisa li-peptide tse tloaelehileng tse ngotsoeng ka tšepe ea lanthanide e le mofani
Bala haholoanyaneLi-peptide tsa TR-FRET
Lidae tsa Fluorescent
Re hlahisa li-peptide tse tloaelehileng tse ngotsoeng ka tšepe ea lanthanide e le mofani
Bala haholoanyaneMokhoa o ikhethileng oa peptide synthesis
Re fana ka li-peptide tse ngotsoeng ka dae ea fluorescent ka sehlopheng sa lipatlisiso kapa e le lisebelisoa tse tala tsa bohlokoa.
