Liphetoho tsa Peptide
Phosphorylation ea peptide
Phosphorylation ea peptide ke eng?
Cellular phosphorylation ke phetoho e ka fetolang ts'ebetso, thepa e tlamang, kapa sebaka sa cellular sa liprotheine kapa li-enzyme. Hape ke ts'ebetso ea bohlokoa ea taolo e phethang karolo ea bohlokoa ho li-pathologies tse ngata tsa mafu, ho kenyeletsa ho ruruha, kankere, mathata a methapo ea kutlo le metabolism. Li-Enzyme tse phosphorylate liprotheine masala a itseng a amino acid (mohlala, tyrosine, serine, le threonine) li bitsoa kinase. Li-enzyme tse tlosang sehlopha sa phosphate ka hydrolysis li bitsoa phosphatase. Hobane phosphorylation e bohareng ba litsela tse ngata tsa ho fetisetsa lipontšo, li-peptide tsa phosphorylated (i.e., phosphopeptides) E sebelisoa bakeng sa tlhahlobo ea protheine kinases le phosphatase litekong tsa cellular tsa may.
Typical location of phosphoryl groups
Phosphoralation e ka hlaha ka Serine (S, Ho ba), Threonine (T, Thr) le Tyrosine (Y, Tyr) liketane tse lehlakoreng ka ho theha bond ea phosphoester. CPC Scientific can synthesize phosphopeptides and incorporate single or multiple combinations of phosphoserine (pS), phosphothreonine (pT), or phosphotyrosine (pY).
Phosphoserine
Phosphoserine is a short peptide or peptide chain containing a phosphorylated serine (pSer), i.e., a peptide fragment in which the hydroxyl group (-OH) of a serine residue is modified by a phosphate group (-PO₄²-).
Phosphothreonine
Phosphothreonine is a short peptide or polypeptide chain containing phosphothreonine (pThr), i.e. a peptide in which the hydroxyl group (-OH) of a threonine residue is modified by a phosphate group (-PO₄²-).
Phosphotyrosine
Phosphotyrosine is a short peptide or polypeptide chain containing phosphotyrosine (pTyr), i.e. a peptide in which the phenolic hydroxyl group (-OH) of a tyrosine (Tyr) residue is modified by a phosphate group (-PO₄²-).
Litlhaloso tse hlahang
PRMT5 C-terminal Phosphorylation e Fetola 14-3-3/PDZ Interaction Switch*
PRMT5 ke enzyme e ka sehloohong e ikarabellang bakeng sa ho behoa ha symmetric dimethylarginine liseleng tsa mammalian.. Ka boiteko ba ho utloisisa hore na PRMT5 e laoloa joang, re khethile sebaka sa threonine phosphorylation ka har'a mohatla oa C-terminal motif, e lebisitsoeng ke Akt/serum- le glucocorticoid-inducible kinases. Ha ho ntse ho batlisisa mosebetsi oa phetoho ena ea posttranslational, re fumane ka mokhoa o khutsitseng hore mohatla oa eona oa mahala oa C-terminal o kopanya libaka tsa PDZ (ha e se na phosphorylated) le 14-3-3 liprotheine (nakong ea phosphorylated). Hantle-ntle, ketsahalo ea phosphorylation ka har'a masala a seng makae a ho qetela a mohatla oa C-terminal e hlahisa PDZ / 14-3-3 e itšetlehileng ka "switch" ea posttranslational. The C-terminal motif ea PRMT5 ea hlokahala bakeng sa ho kopana le membrane ea plasma, 'me tahlehelo ea matla ana a ho fetola ha e tsamaellane le bophelo. Ketsahalo ena ea pontšo e sa tsoa tlalehoa bakeng sa HPV E6 oncoprotein empa ha e e-so bonoe bakeng sa liprotheine tsa mammalian.. Ho batlisisa ho ata ha PDZ / 14-3-3 ho fetola phetisetso ea matšoao, re thehile protheine domain microarray e nang le libaka tsa PDZ le 14-3-3 liprotheine. Re sebelisitse microarray ena ho hlahloba mehatla ea C-terminal ea sehlopha se senyenyane sa liprotheine tsa bonkgetheng le ho tsebahatsa ERBB4., PGHS2, le IRK1 (hammoho le E6 le PRMT5) joalo ka ho tsamaellana le mokhoa ona oa matšoao, ho fana ka maikutlo a hore phetoho ea PDZ/14-3-3 e ka ba paradigm e pharaletseng ea baeloji.
Litšebeletso tse Amanang tsa Phetoho ea Peptide
glycosylation ea peptide
glycosylation ea peptide
Peptide glycosylation ke phetoho e kopanetsoeng e ka ntlafatsang thepa ea physicochemical ea li-peptide.
Bala haholoanyanePhosphorylation ea peptide
Phosphorylation ea peptide
Phosphoralation e ka hlaha ka Serine (S, Ho ba), Threonine (T, Thr) le Tyrosine (Y, Tyr) liketane tse lehlakoreng ka ho theha bond ea phosphoester
Bala haholoanyaneLi-peptide tse tloaelehileng
Li-peptide tse tloaelehileng
Kenyelletso ea li-amino acid tse peli tseo e seng tsa tlhaho tse nang le α-methyl, lihlopha tsa α-alkenyl nakong ea motsoako o tiileng oa liketane tsa peptide.
Bala haholoanyanePeptide ea cyclic
Peptide ea cyclic
Peptide cyclization e ntlafatsa botsitso ba li-peptide (e amanang le li-analogue tsa bona tsa mela) 'me ke leano le tloaelehileng ho nts'etsopele ea peptide.
Bala haholoanyanephetoho ea peptide
Boiphihlelo bo pharalletseng ba phetoho ea peptide, ho fana ka mekhoa e mengata e sebetsang ea lipatlisiso tsa peptide.
