Lisebelisoa
E kopantsoe ho romelloa sebakeng se hloekileng.
Sehlopha 100 kamore e hloekileng e phahameng haholo
Ho tloha mohatong oa pele qalong ea teko ho isa mohatong oa ho qetela oa ho paka, e lula e bolokoa ka nyopa le tikoloho e hloekisitsoeng.
Ea iketsang ho lemoha le taolo: Ho nyopa; khatello ea moea; mongobo; mocheso; likaroloana tsa lerōle li nkoa ka mokhoa o itekanetseng le ho laoloa.
- Ho itšehla thajana ka makhetlo a mabeli
- Ho sefa moea oa HEPA
- E ntle sebaka sa khatello
- Ho Sterilization ka Moetso
- Tlhokomelo ea likaroloana tsa lerole
- Taolo ea mocheso le mongobo
Peptide synthesis
The MOL Changes peptide synthesis lab e na le lisebelisoa tse ngata tse fapaneng tsa synthesis, ho kenyelletsa le boholo bo bongata ba li-reactors le li-tubes tse tiileng tsa phase synthesis ho fihlela 60 li-amino acid ka bolelele.
Liphetoho tsa MOL’ litsebi tsa peptide li ka khona habonolo kopanya le ho fetola li-peptide tse rarahaneng tse kang li-peptide tsa FRET, cyclic kapa stapled peptides, li-peptide tse nang le li-amino acid tse ngata tse sa tloaelehang, joalo joalo.
Ho hloekisa peptide
Laebrari e ruileng ea mekhoa ea tlhoekiso ka katleho e ntlafatsa ho tlosoa ha masala (mohlala. li-amino acid tse sa sebetseng, likhechana tse khutšoane tsa peptide, tshireletso ka lihlahisoa, masala a resin, etc.) ho fumana li-peptide tsa bohloeki bo phahameng.
Mefuta e fapaneng ea lisebelisoa tsa tlhoekiso e tsoetseng pele ke ea bohlokoa haholo ts'ebetsong ea tlhoekiso, mme re sebelisa HPLC ea ho itokisa; evaporator ea rotary; le cryo-centrifuge ho fihlela sepheo sa tlhoekiso.
Peptide lyophilization
Ka mor'a synthesis kapa tlhoekiso, hangata li-peptide li na le TFA (asiti trifluoroacetic), acetonitrile, DMSO, etc. Lyophilization e ka tlosa li-solvents tse setseng mme ea fokotsa phello ea chefo litekong tse latelang.. Holim'a moo, lyophilization ka sebelisoa ho lokisetsa lyophilized phofo bakeng sa peptide quantification le tšebeliso, 'me e ka hlophisoa ka nepo ho tsepamisa mohopolo o hlokahalang (mohlala, e qhibilihisoa ka PBS, metsi a mangata). Ts'ebetso ea lyophilization e boetse e etsoa ka mocheso o tlase, ho fokotsa ho chesoa ha li-peptide tse sa utloeng mocheso (mohlala, li-peptide tse nang le libonto tsa disulfide kapa lihlopha tse fetolang).
Liphetoho tsa MOL li na le mefuta e mengata ea lisebelisoa tsa lyophilization ho tloha ho sefofane ho ea ho sekala, e kopantsoe le ts'ebetso e iketselitseng ea lyophilization e nang le litekanyetso tse thata haholo ho tloha pele ho leqhoa ho isa ho sublimation le annealing., ho fana ka tharollo e ntlafalitsoeng bakeng sa li-peptide tse nang le thepa e fapaneng.
Teko ea Peptide
Nakong ea ts'ebetso ea liteko, mohato o mong le o mong o lokela ho nka liphetho tsa data e le motheo oa ho ntšetsa pele mosebetsi oa liteko ho netefatsa hore liphetho tsa ho qetela tsa lipatlisiso li lumellana.
MOL Chnges e na le mefuta e mengata ea lisebelisoa tse nepahetseng joalo ka mass spectrometry; chromatography ea mokelikeli e phahameng ea ts'ebetso; limithara tse mongobo; li-incubators tsa baktheria; Bahlahlobi ba PH, etc., e re lumellang ho laola boima ba limolek'hule, dikahare, bohloeki, nyopa, metsi, le liphetho tse ling tsa lihlahisoa tsa rona.
Libaka tse ngata tsa QC
Teko le tlhahlobo ea thepa e tala, Taolo ea mahareng ea sehlahisoa, Litlhaloso tsa ho qetela tsa sehlahisoa
