Liphetoho tsa Peptide
Teko ea Peptide
Karolo ea tlhahlobo ea peptide
Teko ea peptide e etsa qeto ea bohloeki, boima ba molek'hule, tatellano e nepahetseng, le boteng ba litšila tsa peptides. Sena se bohlokoa bakeng sa liteko tsa lipatlisiso tsa mahlale, ntshetsopele ya lithethefatsi le likopo tsa bongaka hobane: li-peptide tsa bohloeki bo phahameng (mohlala. >95%) e ka fokotsa tšitiso ea liteko le ho ntlafatsa ts'epahalo ea data. Li-peptide tse sa hloekang li ka ama ts'ebetso ea tlhaho kapa tsa baka karabelo ea 'mele ea ho itšireletsa mafung (mohlala. litšila tse teng lithethefatsing li ka baka ho kula kapa chefo).
Litlhahlobo tse sebelisoang hangata:
HPLC (Chromatography e Phahameng ea Ts'ebetso e Phahameng): e sekaseka bohloeki ba peptide le sebopeho sa litšila.
Mass Spectrometry (MOF): Etsa qeto ea boima ba limolek'hule 'me u netefatse ho nepahala ha tatellano.
Tlhahlobo ea amino acid (AAA): quantitatively fumana sebopeho sa amino acid ea peptide.
Netefatso ea ts'ebetso ea baeloji le ts'ebetso ea li-peptide
Etsa bonnete ba botsitso ba li-reagents tsa peptide
Netefatsa hore sterility le endotoxin e latela melao
Teko e Tloaelehileng ea Peptide
Haholo-holo e kenyelletsa bohloeki ba li-peptide; tlhahlobo ea litaba; tlhahlobo ea sterility; PH boleng; teko ea mongobo; tlhahlobo ea solubility le likarolo tse ling.
Ho beleha
Mokhoa oa ho enta ka kotloloho: enta mecheng ea litaba ka bobeli, qhoela bakeng sa 14 matsatsi 'me u shebelle kholo ea likokoana-hloko.
Mokhoa oa ho sefa li-membrane: Tharollo e ne e fetisoa ka lera le hloekisitsoeng le hloekisitsoeng (pore boholo 0.22 μm), likokoana-hloko li ne li qabeletsoe lera, se ileng sa fetisetsoa sebakeng sa setso bakeng sa ho fuama.
ATP bioluminescence: Ho fumanoa ha ATP e hlahisoang ke microbial metabolism, ka potlako (lihora) empa e hloka netefatso.
Bohloeki
RP-HPLC: Ho ipapisitsoe le phapang ea hydrophobicity ea li-peptide, separation on a C18/C8 column and detection by UV or MS.
MOF: Determination of peptide molecular weight (MALDI-TOF or ESI-MS), verification of agreement with theoretical values and detection of modifications or impurities.
Amino acid analysis principle: after acid hydrolysis of the peptide, quantify the composition of each amino acid and verify the purity by comparing with the theoretical value.
SDS-PAGE: Separation by electrophoresis and observation of single bands by Caulmers Brilliant Blue or Silver staining.
dikahare
Elemental analysis: By decomposing complex physical processes into many tiny “meta-processes”, each “meta-process” follows the same law, the problem is simplified by analyzing these “meta-processes” and applying necessary mathematical methods.
Conservation of mass: Ka karabelo efe kapa efe ea 'mele kapa ea lik'hemik'hale, kakaretso ea bongata ba lintho tse amehang karabelong e lekana le kakaretso ea boima ba lihlahisoa ka mor'a karabelo.
Sebaka se tloaelehileng:Ona ke mokhoa o hlalosoang ka lipalo e le ho fetolela boleng bo felletseng ba sistimi ea 'mele hore e be kamano ea boleng bo lekanyelitsoeng.. Ka ho tloaeleha, lipalo li ka nolofatsoa 'me boleng bo ka fokotsoa ka katleho.
Teko ea Peptide
Fumana mantsoe a qotsitsoeng a tlhahlobo ea peptide ho latela ka nepo bokhoni ba ts'ebetso ea hau ea liteko.
