Liphetoho tsa Peptide
Tobetsa Chemistry Peptides
What is Click Chemistry Peptides?
Click peptides are peptides synthesised using click chemistry. This technology is characterised by high yield, good selectivity, carbon hetero bond binding, wide applicability and easy operation. Click reaction is a key step in peptide chemistry for peptide synthesis and chemoselective modification. Click reactions have a wide range of applications, including ligation, cyclisation, biocoupling and radiolabelling.
The most common functional groups in click chemistry are azides and alkynes. Karabelo ea Azide-alkyne cycloaddition ke karabelo e atileng haholo ea ho tobetsa. Tobetsa k'hemistri e na le mefuta e mengata ea likopo ho motsoako oa lik'hemik'hale, mahlale a thepa, baeloji ea lik'hemik'hale le nts'etsopele ea lithethefatsi.
Peptide Click chemistry e boetse e sebelisoa bakeng sa ho ngola biomolecular, e khethang haholo covalent sehokelo ho libaka shebiloeng, le biocoupling lithutong tsa proteomics. Ka nts'etsopele ea lithethefatsi tsa peptide maemong a mahlale le a bongaka, Click peptides li ntse li lokisoa ka theko e tlase le ka botsitso bo phahameng.
Tobetsa Chemistry Peptides typology
CuAAC le k'hemistri ea mahala ea koporo
CuAAC (koporo-catalysed azide-alkyne cycloaddition) tobetsa ho arabela ho tsoela pele ka ho tobetsa li-peptide tse fetotsoeng tsa alkyne le limolek'hule tse fetotsoeng tsa azide ho qala ka bond ea triazole.. Khemistri ea mahala ea koporo ke mokhoa o mong oa ho tobetsa k'hemistri e nang le tšitiso e tlase ea matla a ts'ebetso mme e sa hlokeng ho tlola hoa cytotoxic ea sesebelisoa sa tšepe..
Karabelo ea CuAAC e kenyelletsa ho thehoa ha litho tse hlano tse thata tsa heterocyclic 1,2,3-triazoles tlas'a maemo a bonolo.. Mabapi le tshebetso e amehang, li-azides li bonolo ho li hlahisa, e tsitsitseng tlasa maemo a metsi le oxidising ebile e sebetsa haholo. Li-triazole tse joalo li lekana le elektroniki tsa bond ea peptide mme li na le polane e ts'oanang le karolo ea amide., empa ha li kotsing ea ho robeha ha hydrolytic.
Thepa tse kang botsitso bo phahameng, ho tšoana le litlamo tsa amide, 'me ts'ebetso e ka bang teng ea baeloji e etsa hore bond ea triazole e se be feela sehokelo se bonolo le se entsoeng habonolo, empa hape ke karolo ea bohlokoa ea k'hemistri ea ho tobetsa.
Sehokelo sa BCN
Lihlopha tsa BCN li ka itšoara ka li-biomolecules tse ngotsoeng ka azide ntle le tlhokahalo ea copper catalyst.BCN linkers li ka kopanngoa le lihlopha tse fapaneng tse sebetsang tse kang lihlopha tsa amine., lihlopha tsa amino, lihlopha tsa aminooxy, lihlopha tsa acid, Lihlopha tsa ester tsa NHS joalo-joalo.
Sehokelo sa DBCO
Lihokela tsa DBCO li ka sebetsana le li-biomolecules tse fetotsoeng ka azide ntle le tlhoko ea li-catalyst tse chefo., ho etsa hore li-reagents tsa DBCO e be tse loketseng ho sebelisoa liseleng le linthong tse phelang.
Sehokelo sa Cyclopropene
Cyclopropene ke motsoako o monyane ka ho fetisisa oa unsaturated carbocyclic o nang le tlatsetso e phahameng, cycladition, le reactivity ea ho bula lesale.
Sehokelo sa TCO
Lihokela tsa TCO ke li-biological orthogonal agents tsa tetrazine. Karabelo ea cycloaddition lipakeng tsa tetrazine le trans-cyclooctene (TCO) e sebelisoa haholo ho bioconjugation, litšoantšo tsa limolek'hule le tlhahlobo ea lisele.
Lihokelo tsa Tetrazine
Tetrazine linker ke bioorthogonal ligand bakeng sa TCO le cyclopropene. Li-link tsa Tetrazine li na le chemoselective 'me li arabela ka ho khetheha ka trans-cyclooctene (TCO). Ke lisebelisoa tse loketseng bakeng sa biocoupling, boenjiniere ba lisele le lithuto tsa khokahano ea sebaka / sepheo se ikhethileng.
Litlhaloso tse hlahang
Phetoho ea Nanostructures ea DNA e nang le Li-Peptide Tse Sebetsang Ka Copper-Free Click Chemistry
Protocol e fanoeng e hlalosa mokhoa oa ho kopanya peptide e fetotsoeng ea DNA ea matsoho a mararo.. Protocol e fana ka lintlha tsa ts'ebetso ea limolek'hule tse tharo tsa DNA tse nang le karolo e le 'ngoe ea amino-modified e nang le peptide e fetotsoeng ea azide., denoted "PeB," ho sebelisa k'hemistri e sa lefelloeng ea koporo. Peptide ena ea PeB, e nkiloeng ho sethibela-mafu se fokolang HC19, ka katleho e hlasela kokoana-hloko ea ntaramane A. Kamora moo, libopeho tsa DNA tse sebetsang hantle tsa peptide li kopantsoe ka sebopeho sa DNA ea matsoho a mararo, ho nolofalletsa tlhahiso e ngata ea li-peptide ho ntlafatsa ho tlama le ho thibela likaroloana tsa kokoana-hloko. Le hoja protocol ena e hlalosa ka ho hlaka ho kopanngoa ha likarolo tse bonolo tsa DNA-peptide, mokhoa o akaretsang o ka lekanyetsoa ho khabisa li-nanostructures tse rarahaneng tsa DNA tse kang tse entsoeng ka tile- kapa meaho e thehiloeng ho origami.
Litšebeletso tse Amanang tsa Phetoho ea Peptide
glycosylation ea peptide
glycosylation ea peptide
Peptide glycosylation ke phetoho e kopanetsoeng e ka ntlafatsang thepa ea physicochemical ea peptide.
Bala haholoanyanePhosphorylation ea peptide
Phosphorylation ea peptide
Phosphoralation e ka hlaha ka Serine (S, Ho ba), Threonine (T, Thr) le Tyrosine (Y, Tyr) liketane tse mahlakoreng ka ho theha bond ea phosphoester
Bala haholoanyaneLi-peptide tse tloaelehileng
Li-peptide tse tloaelehileng
Kenyelletso ea li-amino acid tse peli tseo e seng tsa tlhaho tse nang le α-methyl, lihlopha tsa α-alkenyl nakong ea motsoako o tiileng oa liketane tsa peptide.
Bala haholoanyanePeptide ea cyclic
Peptide ea cyclic
Peptide cyclization e ntlafatsa botsitso ba li-peptide (e amanang le li-analogue tsa bona tsa mela) 'me ke leano le tloaelehileng ho nts'etsopele ea peptide.
Bala haholoanyanephetoho ea peptide
Boiphihlelo bo pharalletseng ba phetoho ea peptide, ho fana ka mekhoa e mengata e sebetsang ea lipatlisiso tsa peptide.
