Seo Thermo Fisher's New Immunopeptidomics Kit e se Bolelang bakeng sa Ts'ebetso e Tloaelehileng ea Peptide Workflows
Mass spectrometry-based immunopeptidomics e fetohile ho tloha sesebelisoa se khethehileng sa ho sibolla thuto ho ba tšiea ea bohlokoa ea bioanalytical bakeng sa biopharmaceutical R.&D. Ho tsoa ho sibollo ea ente ea mofetše oa neoantigen le T-cell receptor (TCR-T) boitsebahatso ba sepheo ho profiling ea sepheo sa autoimmune, tlhahlobo e tobileng ea antigen ea leukocyte ea motho (HLA)-li-peptide tse tlanngoeng li fana ka bopaki bo se nang mohlala ba tlhahiso ea antigen. Leha ho le joalo, ka nalane, ho fapana ha ho hloekisoa ha immunoaffinity (IP), ho itšehla thajana ha peptide ea micro-scale, le ho hloekisoa ha lisampole ka letsoho ho na le meeli e matla haholo ea ho ikatisa ka har'a laboratori le tlhahlobo ea tlhahlobo.
Khoebo ea li-platform tsa ho itokisa tsa sampole tsa immunopeptidomics - tse bontšitsoeng ke Thermo Fisher Scientific's Pierce MHC Class I Antibody Coupling le Immunoprecipitation Kit e kopantsoeng le li-ultra-high-resolution Orbitrap Exploris le Orbitrap Astral LC-MS/MS litsamaiso tsa ts'ebetso.. Ka ho emisa sethibela-mafu sa pan-MHC Class I (setšoantšo sa W6/32) ho kopanya lifaha tsa makenete, phokotso e tlase ea pH, le ho etsa desalting ea C18 ka boiketsetso, li-coefficients tsa tlhahlobo tse tlase tsa mefuta e fapaneng (CV) li theohile ka tlase 15%.
Leha ho le joalo, joalo ka ha sampole ea "lowstream mass spectrometry prep" e fetoha tloaelo le ho ikatisa haholo, ho hlaha bothata bo bocha ba tlhahlobo: Litlhaloso tsa peptide tsa maiketsetso tse holimo. Peptide
joalo ka ha sampole ea "lowstream mass spectrometry prep" e fetoha tloaelo le ho ikatisa haholo, ho hlaha bothata bo bocha ba tlhahlobo: Litlhaloso tsa peptide tsa maiketsetso tse holimo.
Li-kits tse tloaelehileng li phahamisa litebello tsa litekanyetso tsa litšupiso tsa maiketsetso, li-peptide tsa calibration tsa nako ea ho boloka, le isotope e boima e ngotsoeng (SILE) spike-in controls. Ha lisampole tsa ho itokisa li ntse li fokotsehile, synthetic peptide heterogeneity-joalo ka ntlafatso e sa fellang ea isotopic, litaba tse sa nepahalang tsa peptide, tšitiso ya kgahlano, le hydrophobic aggregation-e fetoha mohloli oa mantlha oa phoso ea palo le netefatso ea sepheo se fosahetseng.
Tlhahlobo ena e lekola hore na lisebelisoa tse emeng tsa immunopeptidomics li fetola litlhoko tsa peptide tse holimo joang, e lekola likarolo tsa bohlokoa tsa taolo ea boleng tse hlokahalang bakeng sa ho tsamaellana le lisebelisoa, 'me e hlalosa liphetoho tse sebetsang tseo bafani ba peptide synthesis ea tloaelo ba tlamehang ho li amohela ho tšehetsa lipatlisiso tsa sejoale-joale tsa proteomics.
Tekanyetso ea Boitokisetso ba Mohlala o Motheong: Thepa ea Pierce MHC Kit Benchmark
Ho utloisisa hore na hobaneng lintlha tsa peptide tse holimo li tlameha ho ikamahanya le maemo, Lieta tsa bioanalytical li tlameha ho qala ka ho sekaseka k'hemistri le mechini ea lisebelisoa tse emeng tlase ho noka. Ho latela nalane, immunopeptidomics e itšetlehile ka Sepharose kapa likholomo tsa agarose tsa Protein A/G tse hlokang ho hokahana ka letsoho le dimethyl pimelimidate. (DMP), lihlatsoa tsa matla a khoheli a mangata, le pukwanana sethaleng-tip desalting. Mekhoa ena ea matsoho e hlahisitse phapang e kholo ea ho matha le maemo a phahameng a litšila.
Lisebelisoa tsa khoebo li tsamaisa le ho etsa hore phaephe ena e be e laoloang, mehato e mene ea li-micro-scale protocol e ntlafalitsoeng bakeng sa li-lysate tsa lisele tse kenyang chelete e tlaase (10⁷ ho isa ho 10⁸ lisele) kapa li-tissue biopsy:
E Eketsehileng Immunopeptidomics LC-MS/MS Pipeline
- Khokahano ea Li-antibody: Sesole sa 'mele sa HLA sa Sehlopha sa I sa pan-specific anti- human (setšoantšo sa W6/32) e kopantsoe ka ho toba le lifaha tsa magnetic agarose tsa Protein A/G ka tekanyo e tsitsitseng (2.5 µg antibody ka 1 µL mokelikeli oa lifaha), ho felisa ts'ebetso ea resin ea letsoho le ho feto-fetoha
Sekhechana 176-191 Peptide Phaello e tlase ea pH: Li-complex tsa HLA li khaotsoe 'me li hlakisoa ho sebelisoa maemo 1% asiti trifluoroacetic (TFA) kapa hlapolla 0.1 Ke asiti ea acetic, ho lokolla tlamme 8- ho li-peptide tsa 11-mer haufi le liketane tse boima tsa HLA le beta-2 microglobulin (β2M).
r digito).
- Phaello e tlase ea pH: Li-complex tsa HLA li khaotsoe 'me li hlakisoa ho sebelisoa maemo 1% asiti trifluoroacetic (TFA) kapa hlapolla 0.1 Ke asiti ea acetic, ho lokolla tlamme 8- ho li-peptide tsa 11-mer haufi le liketane tse boima tsa HLA le beta-2 microglobulin (β2M).
- C18 Micro-Desalting & LC-MS/MS: Li-peptide li qheletsoe ka thoko ho liprotheine tse boima ba limolek'hule tse sebelisang malebela a C18 spin kapa malebela a automated microfluidic stage., eluted in 25% ho 50% acetonitrile (ACN) e nang le 0.1% TFA, omisitsoe, botjha ka 0.1% asiti ea formic (FA), le ho hlahlobisisoa ka UHPLC ea boemo bo phahameng bo fetotsoeng bo kopantsoeng le li-spectrometer tsa tandem.
Key Takeaway: Li-kits tse emeng li felisa ho hlophisoa ha likholomo tsa IP, ho fokotsa phapang ea ho ts'oaroa ha sampole ho < 15% CV. Ka lebaka leo, liphoso tsa tlhahlobo ho litlhahlobo tse lebisitsoeng tsa PRM/DIA joale li simolohile haholo-holo ho tsoa ho litekanyetso tsa referense tsa maiketsetso tsa peptide..
Joalo ka ha ho hlalositsoe ka botlalo lipatlisisong tse hatisitsoeng ho PMC's optiPRM Targeted Immunopeptidomics Workflow (2024), lisampole tsa othomathike le tse tloahelehileng ka kit li thusa ho lekola karabelo e tšoanang haholo (PRM) le phumano e ikemetseng ea data (MONO). Leha ho le joalo, ho fihlella palo e felletseng ho hloka maemo a maiketsetso a "AQUA peptide" a ngotsoeng ka boima ao k'hemik'hale ea ona e ts'oanang le ho nepahala ha spectrometer ea boima..
Liphello tse ka holimo tsa Ripple: Ho Hlalosa bocha Litlhaloso tsa Peptide tse Tloaelehileng
Kamohelo e atileng ea lisebelisoa tse emeng tsa immunopeptidomics e fetola litlhaloso tse hlokahalang tsa lik'hemik'hale le tsa 'mele tsa li-peptide tsa maiketsetso tse tloaelehileng ho pholletsa le litekanyo tse nne tse fapaneng.: tatellano-boemo ba ho qhibiliha, bohloeki ba isotopic, netefatso ea litaba tsa peptide, le taolo ea li-counter-ion.
(Drives Demant For) Quantitation Rigor: Net peptide content ka Amino Acid Analysis (AAA) Counter-Ion & Ho beleha: TFA-mahala phapanyetsano & Sehlopha 100 tlhahiso ea kamore e hloekileng
TLHALOSO EA UPSTREAM PEPTIDE RIPPLE Downstream Standardization (Thermo Pierce MHC Kit) Phaello e tlase ea pH (1% TFA) & C18 Micro-Cleanup Orbitrap LC-MS/MS Quantification (PRM/HE) Upstream Custom Custom Peptide Litlhokahalo Hydrophobic Sequence Handling: Anchor-aware synthesis bakeng sa 8-11mers Isotopic Purity: > 99.0% kenyeletso e boima ea isotopo (13C/15N)
1. Tsamaisa Hydrophobic Anchor Sequences le Aggregation
Limolek'hule tsa MHC Class I ka tlhaho li hlahisa li-peptide tse khutšoane, ka tlwaelo 8 ho 11 li-amino acid ka bolelele, e khetholloang ka mekga e thata ya sebopeho. Boemo 2 (P2) le boemo ba C-terminal (P9/P10) e sebetsa e le masala a ankora a mantlha a emang ka har'a lipokothong tse ikhethileng tsa HLA heavy chain binding groove.. Bakeng sa alleles tse tloaelehileng joalo ka HLA-A*02:01, liankora tsena li na le hoo e batlang e le li-amino acid tse nang le hydrophobic aliphatic kapa monko o monate.: Leucine (Leu), Valine (Val), Isoleucine (Ka), Methionine (Kopana), Phenylalanine (Phe), le Tyrosine (Tyr).
Ha li-peptide tsa tloaelo li entsoe li bonahatsa li-endogenous HLA ligands, litaba tsa bona tse phahameng tsa hydrophobic li lebisa ho se nang metsi a mangata haholo le papatso e potlakileng e sa tobang ho polypropylene microcentrifuge tubes le LC autosampler vials..
- Insolubility in Loading Buffers: Li-buffers tse tloaelehileng tsa LC-MS (0.1% FA / 1% ACN) hangata e hloleha ho solubilize hydrophobic 9-mers, ho baka pula e ngata pele ho ente ya kholomo.
- Litahlehelo tsa Adsorption ea Bokaholimo: Likarolong tsa mahloriso tsa picomolar kapa femtomolar tse sebelisetsoang LC-MS spike-in calibration curves., tatellano ea peptide e sa tsitsang e adsorb maboteng a setshelo, khopamisa linearity (R² < 0.95).
2. Isotopic Purity vs. Mamello ea Phoso ea Boholo
Bakeng sa litlhahlobo tse lebisitsoeng tsa immunopeptidomics tse sebelisang Selected Reaction Monitoring (SRM), Parallel Reaction Monitoring (PRM), kapa DIA e nang le maemo a kahare, li-peptide tsa maiketsetso li entsoe ho sebelisoa Stable Isotope e Ngotsoeng (SILE) li-amino acid. Ka tloaelo, boima Arg (13C6, 15N4; phetoho e kgolo +10 Le) kapa boima Lys (13C6, 15N1; phetoho e kgolo +8 Le) e kenyelelitsoe ho C-terminus.
Ho lisebelisoa tsa boleng bo holimo tse kang Orbitrap Astral kapa Orbitrap Eclipse e sebetsang ho 120,000 ho 240,000 matla a ho rarolla (m/z 200), ho mamella liphoso tse ngata ho thibetsoe ho ≤ 5 ppm. Matlafatso e sa phethahalang ea isotopic nakong ea selelekela sa amino acid e baka "ho lutla ha isotope" - mefuta e sa ngolisoeng ea peptide e teng ka har'a maemo a ngotsoeng ka thata.. Haeba ntlafatso ea isotopic e oela ka tlase 99.0%, lets'oao la leseli le setseng le fetana le li-ligand tsa HLA tse ngata haholo, ho hlahisa matshwao a motheo a quantification ya bohata.
3. Net Peptide Content: Ho Fetela ka Ntle ho HPLC Optical Purity
Maikutlo a fosahetseng mabapi le theko ea peptide e tloaelehileng e lekana le bohloeki ba tlhahlobo ea RP-HPLC (214 nm peak area percentage) e nang le peptide e felletseng.
Bohloeki ba RP-HPLC bo bontša feela bongata bo lekantsoeng ba sepheo sa peptide se
Net Peptide Content (%) = Bongata ba Peptide e Hloekileng Src Peptides Laboratory Tatellano/Kakaretso ea Boima ba Phofo ea Lyophilized × 100
s, joalo ka mongobo o setseng, counter-ions (trifluoroacetate kapa acetate), le matsoai a tlamiloeng. Gross gravimetric boima hangata bo fetelletsa litaba tsa 'nete tsa peptide ka 15% ho 35%.
Net Peptide Content (%) = Boima ba Tatelano e Hloekileng ea Peptide / Kakaretso ea Powder ea Lyophilized Boima × 100
Bakeng sa mekhoa e tloaelehileng ea immunopeptidomics workflows e hlokang palo e feletseng (fmol/µL li-spike-ins), ho itšetleha ka boima bo feteletseng bo omeletseng bo hlahisa tsamaiso ea qu
Moetso o Tloaelehileng oa Mokhahlelo oa Peptide (SPSS) e sebelisa trifluoroacetic acid (TFA) bakeng sa ho petsoha ho tloha ho resin le ho hloekisoa-mohato o khutliselitsoeng. Li-counter-ion tsa TFA tse setseng e le letsoai la trifluoroacetate li ka hatella ionization ea electrospray. (ESI) bokgoni nakong Patlisiso 1 Mofani oa Peptides Tlhahlobo ea LC-MS. Ho feta moo, semelo sa ditshila tsa baeloji (li-endotoxins, DNA ea baktheria e setseng, kapa mesaletsa ea keratin/trypsin) e hlahisoang nakong ea ho paka peptide e ka foufatsa li-detectors tsa Orbitrap, e sebetsang ka meedi ya sub-femtomolar ya ho lemoha.
tse setseng e le letsoai la trifluoroacetate li ka hatella ionization ea electrospray (ESI) katleho nakong ea tlhahlobo ea LC-MS. Ho feta moo, semelo sa ditshila tsa baeloji (li-endotoxins, DNA ea baktheria e setseng, kapa mesaletsa ea keratin/trypsin) e hlahisoang nakong ea ho paka peptide e ka foufatsa li-detectors tsa Orbitrap, e sebetsang ka meedi ya sub-femtomolar ya ho lemoha.
Taolo ea Boleng le Litlhoko tsa Phetoho Bakeng sa Maemo a Ngotsoeng ka Boima
Ho hokahanya tlhahiso ea peptide e tloaelehileng le lisebelisoa tse phahameng, barekisi ba maiketsetso ba tlameha ho nchafatsa Taolo ea Boleng ba bona (QC) meralo le mekhoa ea ts'ebetso ea phano.
Analytical Thresholds for Immunopeptidomics-Ready Custom Peptides
| Paramethara | Sehlopha sa Lipatlisiso se Tloaelehileng | Sehlopha se Lokisitsoeng sa Immunopeptidomics | Mokhoa oa ho netefatsa oa Analytical |
|---|---|---|---|
| RP-HPLC Bohloeki | ≥ 90% | ≥ 98% | C18 RP-HPLC (0.1% TFA / ACN gradient, 214 nm) |
| Ho Nepaha ha Bongata | ± 1.0 Le | ≤ 5 ppm (± 0.005 Le) | E phahameng-Resolution ESI-Orbitrap / MALDI-TOF MS |
| Matlafatso ea Isotopic | > 95% | > 99.0% | Tlhahlobo ea kabo ea enfelopo ea Tandem MS isotopic |
| Net Peptide Content | Ha e lekanngoe (Boima bo felletseng) | Theko e nepahetseng e netefalitsoe ka AAA | Tlhahlobo ea Amino Acid e ngata (AAA) |
| Setifikeiti sa Solubility | Tlhahlobo ea metsi e bonahalang | E netefalitsoe ka 0.1% FA / 1% ACN | Centrifugation & Netefatso ea sebaka se phahameng sa LC-MS |
| Boemo ba Endotoxin | E sa laoleheng | < 0.01 EU/mg | Tlhahlobo ea LAL ea Chromogenic |
| Sebaka sa Tlhahiso | Kakaretso Laboratori | Sehlopha 100 Kamore e Hloekileng ea Ultra-Sterile | ISO 14644-1 Sehlopha 5 tlhokomelo ea tikoloho |
Bohlokoa ba Phetoho ho Neoantigen Screening
Ho motho ka mong kankere ea immunotherapy le nts'etsopele ea ente ea neoantigen, Bafuputsi ba arola li-endogenous HLA ligands ba sebelisa lisebelisoa tse kang Thermo Fisher Pierce MHC Sehlopha sa I Sample Prep Workflow le ho khetholla li-peptide tse fetotsoeng tsa mokhethoa ka hare 48 ho 72 lihora ka hape tatellano ea li-algorithms.
Ho netefatsa mokhethoa oa neoantigens, bafuputsi ba tlameha hang-hang ho odara matching synthetic heavy-labeled (SILE) lilaebrari tsa peptide (ka tlwaelo 20 ho 96 tatelano) bakeng sa netefatso ea PRM. Traditional custom peptide lead times of 4 ho 6 Libeke li baka tšitiso e sa amoheleheng liphaepheng tsa lipatlisiso tsa bongaka. Barekisi ba sejoale-joale ba li-peptide ba tlameha ho fana ka motsoako o tšoanang oa seliba sa 96 le a 5 ho 7 phetoho ea letsatsi la khoebo ntle le ho sekisetsa mekhoa ea tlhahlobo ea QC.
Liphetoho tsa Leano bakeng sa Bafani ba Tloaelehileng ba Peptide: Ho Nolofatsa Kamohelo ea Mofuputsi
Ho thusa bafuputsi ho amohela lisebelisoa tse emeng tsa immunopeptidomics ka kholiseho e felletseng, Bafani ba peptide synthesis ea tloaelo ba tlameha ho tloha ho baetsi ba likonteraka ba sa sebetseng ho ea ho balekane ba khethehileng ba bioanalytical..
Li-platform tsa pele tsa synthesis, joalo ka MOL Liphetoho 'sethala se ikhethileng sa peptide synthesis, bonts'a hore na ho ikamahanya le maemo a tekheniki ho rarolla mathata a lisampole joang.
LITABA TSE THARO TSA TŠEBELETSO TSA BABELI BA PEPTIDE
- Li-Catalogs tse Hlophisitsoeng tsa Immunopeptidomics-Ready Libraries tsa Allele-Specific Reference (HLA-A02:01, HLA-A24:02, HLA-B*0:02) Maemo a Tekanyetso a Nako ea ho Boloka (Metsoako e ngotsoeng ka isotopically ea PRTC-grade)
- Targeted Immunopeptidomics QC Panels High-Res ESI-MS Mass Accuracy (<= 5 ppm) & Matlafatso ea Isotopic (> 99.0%) Setifikeiti sa AAA Net Peptide Content & 1% Teko ea Solubility ea TFA
- SIL e potlakileng ea ho ngola ka boima & Cleanroom Synthesis High-Throughput Parallel 96-Well SPPS (5-7 Fesetere ea Phano ea Letsatsi) Sehlopha 100 Ho Etsa Kamore e Hloekileng ho Felisa Litšilafalo tsa Background MS
1. Li-Catalogs tse Lokisitsoeng tsa Immunopeptidomics
Bafani ba Peptide ba lokela ho fana ka tse entsoeng esale pele, lilaebrari tsa peptide tse hlahlobiloeng esale pele tse etselitsoeng li-supertypes tse kholo tsa HLA hammoho le metsoako ea nako ea ho boloka nako..
- Allele-Specific Reference Libraries: Lihlopha tse entsoeng esale pele tsa li-endogenous ligands tse nang le kamano e phahameng bakeng sa li-allele tsa batho tse atileng (mohlala, HLA-A*02:01, HLA-A*24:02, HLA-B*0:02, le HLA-DRB1*01:01). Tsena li sebetsa e le litsamaiso tse ntle bakeng sa katleho ea ntlafatso ea kit immunoaffinity.
- Metsoako ea Tekanyetso ea Nako ea ho Boloka: Li-cocktails tse ngotsoeng ka boima tsa peptide tse aparetseng spectrum eohle ea hydrophobicity (linako tsa ho boloka likhahla ho tloha 5% ho 65% ACN). E kenngoe ka har'a lisebelisoa tse tsoang pele ho tlhoekiso ea C18, litekanyetso tsena li lumella ho ikamahanya ha nako ea ho boloka nako ka har'a li-multi-batch LC-MS.
2. Liphanele tsa QC tsa Immunopeptidomics tse lebisitsoeng
Ho e-na le ho fana ka Litifikeiti tse tloaelehileng tsa Tlhahlobo (CoAs) e nang le li-trace overlays tsa mantlha tsa HPLC, bafani ba peptide ba tlameha ho hlahisa liphanele tse khethehileng tsa LC-MS QC:
Immunopeptidomics CoA Standard: Peptide e 'ngoe le e 'ngoe e entsoeng e kenyelletsa tekanyo e nepahetseng ea boima ba ESI-MS (≤ 5 ppm), tandem MS isotopic enrichment tiisetso (> 99.0%), AAA-certified net peptide concentration, le netefatso ea solubility ka 0.1% FA / 1% ACN.
Ka ho sebelisa chelete isotope e boima e ngotsweng (SILE) litekanyetso tsa peptide e tšehelitsoe ke litaba tsa AAA tse netefalitsoeng, Bafuputsi ba felisa leeme la mahloriso ha ba theha li-curve tsa calibration tse felletseng ho li-platform tsa Orbitrap LC-MS..
3. Rapid Heavy-Isotope Labeling & Kamore ea ho Hloeka Synthesis
Li-platform tsa tloaelo tsa peptide li tlameha ho kopanya automated parallel Solid-Phase Peptide Synthesis (SPSS) e nang le isotope e tsitsitseng e ngotsoeng amino acid (^{13}C₆, ^{15}N₄-Arg le ^{13}C₆, ^{15}N₁ Lenane).
Ho feta moo, ho thibela litšila tsa tikoloho hore li se ke tsa kena-kenana le ho fumanoa ha li-HLA ligand tse fokolang, tlhaho, tlhoekiso, 'me aliquoting e lokela ho etsahala ka har'a tikoloho e sa hloekang ea tlhahiso. Lisebelisoa tse sebelisang a Sehlopha 100 tikoloho ea kamore e hloekileng le taolo ea boleng ba LC-MS e netefalitsoeng netefatsa khatello ea ion ea mokokotlo le maemo a tlase haholo a endotoxin (< 0.01 EU/mg) e loketse bakeng sa liteko tsa ts'ebetso ea lisele tsa T-cell tse tlase.
Lethathamo la Tekolo ea Barekisi bakeng sa li-Peptide tsa Tloaelo tse Tšoanang le Kit
Ha u lekola barekisi ba peptide ba tloaelo bakeng sa litekanyetso tsa litšupiso tsa immunopeptidomics le lilaebrari tsa SIL, lihlopha tsa thepa ea thepa ea bioanalytical li lokela ho sebelisa matrix a liqeto tse latelang:
| Litekanyetso tsa Tekolo | Mofani oa Peptide ea Tloaelehileng | Immunopeptidomics-Adapted Morekisi (Liphetoho tsa MOL) | Kotsi / Tšusumetso ho Kit Workflow |
|---|---|---|---|
| Tikoloho ea Synthesis | Benche e bulehileng / lab e tloaelehileng | Sehlopha 100 Kamore e Hloekileng ea Ultra-Sterile | Lerata le phahameng la morao-rao la MS & tšilafalo ea endotoxin litlhahlobong tsa lisele |
| Netefatso ea Bohloeki | Sebaka se phahameng sa RP-HPLC (214 nm) | Dual RP-HPLC & HR-ESI-MS (≤ 5 ppm) | Li-peptide tse sa tsejoeng tsa ho hlakolwa li baka tšitiso e phahameng ho likanale tsa PRM |
| Quantitation Motheo | Gravimetric boima ba 'mele | AAA-Certified Net Peptide Content | Ho fihlela ho 35% phoso ea mahloriso ka bongata ba AQUA bo felletseng |
| Matlafatso ea Isotopic | > 95% kakaretso SIL | > 99.0% netefatso ya monono o boima | Ho lutla ha isotope e bobebe ho baka mats'oao a 'nete |
| Tšehetso ea Solubility | Teko ea motheo ea metsi | Teko e ikhethileng ea buffer (0.1% FA / 1% ACN) | Pula ea peptide ka har'a LC autosampler vials e bakang tahlehelo ea matšoao |
| Phepelo Phetoho | 4 ho 6 libeke | 5 ho 7 matsatsi a khoebo (96-liphanele hantle) | E boloka netefatso ea neoantigen le ts'ebetso ea sampole ea bongaka |
Litlhahiso tsa Leano bakeng sa Bafuputsi ba Immunopeptidomics
Ho hlophisoa ha lisampole tsa "mass spectrometry" ka lisebelisoa tse kang Thermo Fisher's Pierce MHC Kit ho bontša mohato o moholo oa ho ea pele bakeng sa lipatlisiso tsa proteomics.. Leha ho le joalo, katleho ea tlhahlobo e ntse e tlamahane haholo le boleng ba li-peptide tsa maiketsetso tse holimo.
Ho eketsa kutloisiso ea tlhahlobo le ho nepahala ha palo:
- Laela AAA-Certified Net Content: Le ka mohla u se ke ua itšetleha ka boima bo feteletseng ba lyophilized bakeng sa li-spike-ins tse ngotsoeng ka thata tsa AQUA peptide. E hloka setifikeiti sa AAA ho netefatsa palo e nepahetseng ea molar.
- Netefatsa Mekhahlelo ea Matlafatso ea Isotopic: Etsa bonnete ba hore li-peptide tse ngotsoeng ka boima li jara > 99.0% bohloeki ba isotopic ho qoba ho kopana ha lets'oao la ketane ea leseli holim'a li-spectrometer tsa Orbitrap mass tsa Ultra-high-resolution.
- Selekane le Bafani ba Khethehileng ba Synthesis: Sebetsa le barekisi ba peptide ba khonang ho hlahisa tatelano ea tloaelo ka har'a Sehlopha 100 tikoloho ea likamore tse hloekileng le ho fana ka thepa ka potlako, lilaebrari tse tšoanang tsa SIL peptide.
Ka ho hokahanya lintlha tsa peptide tsa tloaelo tse holimo le lisebelisoa tse tloaelehileng tsa lisampole tsa prep, Bafuputsi ba ka etsa lithuto tsa immunopeptidomics ka kholiseho e felletseng ea bongata.
Bakeng sa bafuputsi ba batlang lilaebrari tsa peptide tse lumellanang le kit, li-isotope tse boima tse ngotsoeng litekanyetso, kapa liphanele tse lebisitsoeng tsa immunopeptidomics QC, buisana le litsebi tsa bioanalytical ho MOL Changes ho buisana ka bokhoni ba tatellano ea moetlo le likhetho tsa ho kopanya ntlo ea bohloeki.
Mongoli & Tlhahlobo ea Theknoloji
E hlahlobiloe ke MOL Changes Bioanalytical R&Sehlopha sa D
Senior Mass Spectrometry & Litsebi tsa Peptide Synthesis
Tlhahlobo ena e ngotsoe le ho hlahlojoa ke bioanalytical R&D litsebi ho Liphetoho tsa MOL, ho kopanya boitseanape ba mass spectrometry-grade custom peptide synthesis, ho ngola li-isotopi tse tsitsitseng, le tlhahiso ea likamore tse hloekileng bakeng sa lipatlisiso tse nepahetseng tsa immunopeptidomics.
