Myeloid takamaiman ɗan adam CD33 mai karɓa ne mai hanawa tare da bambancin aikin ITIM a cikin ɗaukar phosphatases SHP-1 da SHP-2

CD33 wani memba ne na myeloid na musamman na dangin sialic acid mai ɗaure mai karɓa kuma an bayyana shi sosai akan sel progenitor na myeloid amma a ƙananan matakan a cikin sel daban.. Human CD33 yana da ragowar tyrosine guda biyu a cikin yankin cytoplasmic (Y340 da Y358). Lokacin da phosphorylate, waɗannan tyrosines na iya aiki azaman wuraren docking na phosphatases, SHP-1 da/ko SHP-2, yana ba CD33 damar aiki azaman mai karɓar mai hanawa. Anan mun nuna cewa CD33 shine tyrosine phosphorylated a gaban mai hana phosphatase., mamayewa, kuma ya dauki SHP-1 da SHP-2. Nazarin haɗin gwiwa ya nuna cewa Src-family kinase Lck yana da tasiri a phosphorylating Y340, amma ba Y358, yana ba da shawarar cewa waɗannan ragowar na iya aiki a cikin zaɓin ɗaukar nauyin kwayoyin adaftar kuma suna da ayyuka daban-daban.. Ƙarin tallafi don haɗuwa, amma mara amfani, Matsayi na Y340 da Y358 sun fito ne daga nazarin dauri na peptide wanda ya bayyana daukar ma'aikata na SHP-1 da SHP-2 zuwa Y340 amma SHP-2 zuwa Y358 kawai.. Bincike ta amfani da maye gurbi na SHP-1 ya nuna cewa ɗaure Y340 na CD33 ya kasance da farko ga yankin amino Src homology-2 na SHP-1.. An nuna yuwuwar CD33 don yin aiki azaman mai karɓar mai hanawa ta ikonsa na rage-daidaita CD64-induced calcium mobilization in U937. An nuna dogaro da wannan hanawa akan SHP-1 ta hanyar toshe tasirin tsaka-tsakin CD33 tare da mummunan SHP-1.. Wannan sakamakon yana nuna cewa CD33 mai karɓar mai hanawa ne kuma SHP-1 phosphatase yana da muhimmiyar rawa wajen daidaita aikin CD33.. Ƙarin karatu yana da mahimmanci don gano mai karɓa(s) CD33 ya hana a cikin vivo da aikinsa a cikin ci gaban layin myeloid.