Liphetoho tsa Peptide
Isotope e bitsoang Peptides
Isotope e ngotsoeng ke li-peptides ke eng?
Letšoao le tsitsitseng la isotope ke reagent ea lik'hemik'hale moo limolek'hule tsa motsoako li nang le athomo e le 'ngoe kapa ho feta tse tsitsitseng tsa isotope 'me li hlahlojoa le ho etsoa qeto ka ho sebelisa thepa ea tsona e ikhethang..
Mekhoa e tsitsitseng ea ho ngola li-isotope e thehiloe holim'a molao-motheo oa ho ngola reactant ka ho fetola liathomo tse itseng ka isotope., e ntan'o etsa hore reactant e sebetse 'me e bone liathomo ka phetoho ea nomoro ea neutron., e ka lateloang ke maikutlo, mekhoa ea metabolic kapa lisele. Isotope e tsitsitseng (13C, 15N, 13C15N) li-peptide tse ngotsoeng hangata li sebelisoa e le 'li-tag tsa lik'hemik'hale' ho proteomics ea bongata bakeng sa ho lemoha le ho ts'oara mehlala ea likokoana-hloko.. Mekhoa e mengata ea proteomics haholo-holo e sebelisa "high-resolution mass spectrometry" ho arola li-ion tsa peptide kapa li-ion tsa peptide tse nang le mabitso a isotope., le ho sebelisa matla a mats'oao a bona bakeng sa lipalo tsa palo.
Theknoloji e tsitsitseng ea ho ngola isotopu e na le melemo ea ho utloahala haholo, boemo bo bonolo le quantification e nepahetseng, e etsang hore phetoho ea isotope e fumane tlhokomelo e ngata le ho feta lefapheng la bongaka le biochemistry.
Isotope e bitsoang Peptides
Lisebelisoa tse Nepahetseng bakeng sa Lipatlisiso
Li-isotope tse tsitsitseng tse ngotsoeng li-peptide li entsoe ka ho kenya liathomo tse khethehileng ho li-amino acid tsa tlhaho ka li-isotopes tse boima., joalo ka ho nkela 12C sebaka ka 13C, 14N le 15N, kapa 1H e nang le 2H (deuterium). Liphetoho tsena li fella ka metsoako e ngotsoeng ea isotope e bolokang thepa e tšoanang ea physiochemical le lik'hemik'hale tse tšoanang le tsa tsona tse sa ngolisoang.. Sena se etsa hore e be tsa bohlokoa haholo lits'ebetsong tse ngata tsa saense, ho kenyeletsoa spectrometry ea boima, Lithuto tsa NMR, le tlhahlobo ea pharmacokinetic.
|
Amino Acid
|
Isotope
|
Phapang ea Boima ho AA e sa ngolisoang
|
|---|---|---|
|
Alanine |
U- 13C3, U-15N |
+4 Le |
|
Arginine |
U- 13C6, U-15N4 |
+10 Le |
|
Leucine |
U- 13C6, U-15N |
+7 Le |
|
Lysine |
U- 13C6, U-15N2 |
+8 Le |
|
Phenylalanine |
U- 13C9, U-15N |
+10 Le |
|
Proline |
U- 13C5, U-15N |
+6 Le |
|
Valine |
U- 13C5, U-15N |
+6 Le |
Litlhaloso tse hlahang
Ho sebelisa li-isotope tse tsitsitseng ho ntlafatsa kutloisiso ea rona ea lefu la Alzheimer's etiology le pathology
Leibole le tsitsitseng la isotope le mass spectrometry (MOF)-tlhahlobo ea proteomic e thehiloeng e fetohile leano le matla la ho lekola maemo a boemo bo tsitsitseng ba protheine, tlhahiso ea protheine, le protheine localization. Ho sebelisa sethala sena sa tlhahlobo ho mafu a neurodegenerative ho ka senola likarolo tse ncha tsa etiology ea mafu ana a senyang.. Haufinyane tjena, Li-isotopes-MS tse tsitsitseng li 'nile tsa sebelisoa ho batlisisa mekhoa ea pele ea mafu a lefu la Alzheimer's (AD) ka mefuta ea litoeba tsa lefu la AD-joaloka. Tlhahlobisong ena, re akaretsa meralo ena e tsitsitseng ea liteko tsa isotope-MS le ts'ebeliso ea morao-rao maemong a lefu la AD. Re boetse re hlalosa matsapa a rona a hajwale a reretsweng ho sebedisa nyutlelie magnetic resonance (NMR) tlhahlobo ea isotope e tsitsitseng e ngotsoeng li-amyloid fibrils ho tloha ho AD mouse model brains. Ka kopanelo, mekhoa ena e fana ka menyetla e mecha ea ho ithuta liphetoho tsa proteome ho AD le mafu a mang a neurodegenerative ka ho hlakisa mekhoa ea ho shebisisa kalafo le thibelo..
Litšebeletso tse Amanang tsa Phetoho ea Peptide
glycosylation ea peptide
glycosylation ea peptide
Peptide glycosylation ke phetoho e kopanetsoeng e ka ntlafatsang thepa ea physicochemical ea li-peptide.
Bala haholoanyanePhosphorylation ea peptide
Phosphorylation ea peptide
Phosphoralation e ka hlaha ka Serine (S, Ho ba), Threonine (T, Thr) le Tyrosine (Y, Tyr) liketane tse lehlakoreng ka ho theha bond ea phosphoester
Bala haholoanyaneLi-peptide tse tloaelehileng
Li-peptide tse tloaelehileng
Kenyelletso ea li-amino acid tse peli tseo e seng tsa tlhaho tse nang le α-methyl, lihlopha tsa α-alkenyl nakong ea motsoako o tiileng oa liketane tsa peptide.
Bala haholoanyanePeptide ea cyclic
Peptide ea cyclic
Peptide cyclization e ntlafatsa botsitso ba li-peptide (e amanang le li-analogue tsa bona tsa mela) 'me ke leano le tloaelehileng ho nts'etsopele ea peptide.
Bala haholoanyanephetoho ea peptide
Boiphihlelo bo pharalletseng ba phetoho ea peptide, ho fana ka mekhoa e mengata e sebetsang ea lipatlisiso tsa peptide.
