Haɗin Peptide shine mabuɗin fasaha don samun peptides tare da takamaiman jeri da ayyuka, wanda ake amfani da shi sosai a fagagen nazarin halittu da kimiyyar halitta. Hanyar hada-hadar ruwa ta gargajiya tana da wahala kuma ba ta da ƙarfi, wanda ke da wahala a iya biyan buƙatu da yawa, kuma Bruce Merrifield's Solid Phase Peptide Synthesis ya canza shi (SPSS) in 1963, wanda aka ba shi lambar yabo ta Nobel a fannin Kimiyyar Kimiyya a 1984. Duk da haka, da gargajiya m lokaci kira dabara na bukatar maimaita matakai na deprotection, hadawa da wanka don tsawaita sarkar peptide a hankali, kuma a karshe yanke maƙasudin kwayoyin halitta daga mai ɗauka, wanda bai dace da ra'ayin kimiyyar kore ba. Juya Halin Tsayayyen Mataki na Peptide Synthesis (RP-SPPS) yana amfani da masu ɗaukar hydrophobic (misali, polystyrene resin) da polar kaushi (misali, DMF), wanda shi ne akasin al'ada lokaci m lokaci kira. A cikin 'yan shekarun nan, RP-SPPS an ƙara yin amfani da shi sosai a fagen haɗin peptide saboda fa'idodinsa na musamman..
Amfani
Idan aka kwatanta da na gargajiya gaba m-lokaci kira, baya m-lokaci kira (RP-SPPS) yana da fa'idodi na musamman.
Gaba ɗaya kwaikwayi kirar peptide a cikin kwayoyin halitta: Yana da matukar dacewa da jagorancin kira na peptide a cikin kwayoyin halitta, wanda ke ba da tushen gwaji na in vitro don nazarin peptide da haɗin furotin a cikin kwayoyin halitta.
Mafi sauƙi tsarkakewa: Mai ɗaukar hydrophobic yana da sauƙin wankewa tare da kaushi na halitta, yadda ya kamata ya kawar da wuce kima reagents da by-kayayyakin, kuma yana sauƙaƙa matakan tsarkakewa.
gyare-gyare na musamman na carboxy-terminal: RP-SPPS yana ba da damar gyare-gyaren carboxy-terminal don haɗa kai tsaye akan guduro mai ƙarfi-lokaci., kawar da buƙatar pre-gyara ko gyare-gyare bayan haɗin sarkar peptide.
Ƙari cikin layi tare da koren sunadarai: Juya-lokaci m-lokaci kira yana da juriya ga halayen a karkashin ruwaye yanayi da kuma bukatar kawai karamin adadin kwayoyin kaushi don nagarta sosai kammala hada guda biyu..
Ya dace da dabarun da ba su da kariya: RP-SPPS ya dace da mafi yawan dabarun peptide roba ba tare da kariya ba akan kasuwa, yin peptide kira sauƙi.
Tsawon sarkar Peptide a cikin hanyar N-C
Abubuwan Hakuri
Tare da haɓaka sabbin masu ɗaukar kaya, hada guda biyu reagents da sarrafa kansa synthesizers, RP-SPPS za ta taka rawar gani sosai a fagen haɗin peptide, samar da mafi inganci kuma dacewa hanyoyin haɗin peptide don fannonin biomedicine da kimiyyar kayan aiki.
