Ihe Thermo Fisher's New Immunopeptidomics Kit Pụtara maka Omenala Peptide Workflows
Immunopeptidomics nke dabeere na Mass spectrometry esiwo na ngwa nchọpụta mmuta pụrụ iche abanyela na ogidi bioanalytical dị mkpa maka biopharmaceutical R.&D. Site na nchọpụta ọgwụ mgbochi ọrịa kansa neoantigen na nnabata T-cell (TCR-T) njirimara ebumnuche maka profaịlụ ebumnuche autoimmune, ajụjụ ọnụ nke antigen leukocyte mmadụ (HLA)-peptides agbatị na-enye ihe akaebe na-enweghị ihe ngosi nke ngosipụta antigen. Agbanyeghị, akụkọ ihe mere eme, mgbanwe na immunoaffinity ọcha (IP), mwepu peptide micro-scale, na ntuziaka sample mkpocha nke ukwuu mmachi n'etiti-laboratory reproducibility na nyocha mmepụta.
Azụmahịa nke usoro nkwadebe ihe atụ nke immunopeptidomics ọkọlọtọ - nke Thermo Fisher Scientific's Pierce MHC Class I Antibody Coupling na Immunoprecipitation Kit jikọtara ya na Orbitrap Exploris nke dị elu na Orbitrap Astral LC-MS/MS sistemụ na-egosipụta usoro ntozu oke ọrụ.. Site n'ịhazi pan-MHC Class I antibody (ihe mkpuchi W6/32) ndọta bead njikọ, obere pH elu, na akpaaka C18 desalting, ọnụọgụ nyocha nke mgbada dị iche iche (CV) adaala n'okpuru 15%.
Ma, dị ka downstream mass spectrometry sample prep na-aghọ a na-emekarị na nke ukwuu mmeghari, ọkpọkọ nyocha ọhụrụ na-apụta: nkọwapụta peptide synthetic omenala elu elu. Peptide
dị ka downstream mass spectrometry sample prep na-aghọ a na-emekarị na nke ukwuu mmeghari, ọkpọkọ nyocha ọhụrụ na-apụta: nkọwapụta peptide synthetic omenala elu elu.
Ngwa ndị ahaziri ahazi na-ebuli atụmanya maka ụkpụrụ ntụaka sịntetik, peptides nhazi oge njide, na nnukwu isotope akpọrọ (SIL) njikwa ọnyà. Mgbe a na-ebelata mgbanwe nkwadebe nlele ala, heterogeneity peptide sịntetik - dị ka ịba ụba isotopic ezughị ezu, peptide net ezighi ezi, mgbochi-ion nnyonye anya, na hydrophobic aggregation — na-aghọ isi iyi nke njehie quntitation na ụgha-adịghị mma ebumnuche nkwado..
Nyocha a na-enyocha ka ngwa immunopeptidomics ahaziri ahazi si agbanwe ihe achọrọ peptide omenala elu, na-enyocha oke njikwa mma dị mkpa achọrọ maka ndakọrịta ngwa, ma kọwapụta mgbanwe ndị bara uru nke ndị na-enye njikọ peptide omenala ga-agbaso iji kwado nyocha proteomics ọgbara ọhụrụ.
Ịhazi Nkwadebe Nlereanya N'okpuru: Pierce MHC Kit Benchmark
Iji ghọta ihe kpatara nkọwapụta peptide dị elu ga-agbanwerịrị, Nduzi bioanalytical ga-ebu ụzọ nyochaa kemịkalụ na mekaniki nke ngwa mgbada ala ahaziri ahazi. N'akụkọ ihe mere eme, immunopeptidomics dabere na sepharose juru n'ọnụ ma ọ bụ Protein A/G agarose kọlụm chọrọ ịgafe akwụkwọ ntuziaka na dimethyl pimelimidate. (DMP), multi-nzọụkwụ ike ndọda washes, na ntuziaka ogbo-ndụmọdụ desalting. Usoro ntuziaka ntuziaka ndị a webatara mgbanwe dị ukwuu na-agba ọsọ na ọkwa mmetọ n'okirikiri dị elu.
Ngwa ngwa ahịa na-agbatị ma na-ahazi pipeline a ka ọ bụrụ nke a na-achịkwa, Usoro ụkpụrụ micro-ọnụ ọgụgụ dị nkeji anọ emebere maka lysates cell ntinye dị ala (10sel ruo 10) ma ọ bụ biopsies anụ ahụ:
Ọkọlọtọ Immunopetidomics LC-MS/MS Pipeline
- Njikọta mgbochi: Pan-kpọmkwem mgbochi mmadụ HLA Class I mgbochi mmadụ (ihe mkpuchi W6/32) jikọtara ya na Protein A/G magnetik agarose beads n'ogo a kapịrị ọnụ (2.5 µg antibody kwa 1 µL bead slurry), na-ewepu ntuziaka resin ịgbalite na variabl
Iberibe 176-191 Peptide Elu pH dị ala: A na-ekewa ogige HLA ma gbakọọ ya site na iji ahaziri ahazi 1% trifluoroacetic acid (TFA) ma ọ bụ itughari 0.1 N acetic acid, ntọhapụ agbụ 8- na peptides 11-mer n'akụkụ agbụ HLA dị arọ na beta-2 microglobulin (β2M).
r digiton).
- Elu pH dị ala: A na-ekewa ogige HLA ma gbakọọ ya site na iji ahaziri ahazi 1% trifluoroacetic acid (TFA) ma ọ bụ itughari 0.1 N acetic acid, ntọhapụ agbụ 8- na peptides 11-mer n'akụkụ agbụ HLA dị arọ na beta-2 microglobulin (β2M).
- C18 Micro-Desalting & LC-MS/MS: A na-ekewa Peptides na protein dị arọ dị elu na-eji ndụmọdụ C18 ma ọ bụ ndụmọdụ microfluidic na-akpaghị aka., elu n'ime 25% ka 50% acetonitrile (ACN) nwere 0.1% TFA, akpọnwụwo, emegharịrị na 0.1% formic acid (FA), wee nyochaa ya site na UHPLC ntụgharị ntụgharị dị elu yana spectrometers tandem mass spectrometers..
Ntugharị igodo: Ngwa ndị ahaziri ahazi na-ewepụ nkwadebe kọlụm IP ntuziaka, mbenata ọdịiche njikwa ihe nlere anya na < 15% CV. N'ihi ya, Njehie nyocha na nyocha PRM/DIA ezubere iche ugbu a sitere na ụkpụrụ ntụaka peptide sịntetik sịntetik..
Dị ka nkọwa zuru ezu na nnyocha e bipụtara na PMC's optiPRM Ezubere Immunopeptidomics Workflow (2024), nhazi ihe nlere akpaaka na ngwa ngwa na-enyere nlele mmeghachi omume myirịta aka nke ukwuu (PRM) na nnweta data na-adabereghị na ya (EBE AHỤ). Agbanyeghị, imezu ngụkọ zuru oke chọrọ ụkpụrụ AQUA peptide dị arọ nke sịntetik nke nguzobe kemịkalụ dabara na nkenke nke spectrometer uka..
Mmetụta Ripple dị n'elu: Na-akọwapụta nkọwapụta Peptide omenala
Nkwenye zuru ebe niile nke ngwa immunopeptidomics ọkọlọtọ na-agbanwe kemịkalụ na nkọwa anụ ahụ achọrọ nke peptides synthetic omenala gafee akụkụ anọ dị iche iche.: usoro-larịị solubility, isotopic ịdị ọcha, nkwenye ọdịnaya peptide net, na njikwa counter-ion.
(Na-eme ihe achọrọ maka) Ọnụ ọgụgụ Rigor: Ọdịnaya peptide net site na nyocha Amino Acid (AAA) Counter-ion & Ịgba nwa: Ngbanwe na-enweghị TFA & Klas 100 mmepụta ụlọ dị ọcha
Nkọwapụta UPSTREAM PEPTIDE RIPLE Downstream Standardization (Thermo Pierce MHC Kit) Elu pH dị ala (1% TFA) & C18 Micro-Cleanup Orbitrap LC-MS/MS Quantification (PRM/HE) Ihe chọrọ Peptide omenala na-ejikwa usoro Hydrophobic: Njikọ arịlịka maara maka 8-11mers Isotopic ịdị ọcha: > 99.0% nnukwu isotope incorporation (13C/15N)
1. Na-agagharị Usoro arịlịka Hydrophobic na mkpokọta
MHC Klas I ụmụ irighiri ihe na-enwekarị peptides dị mkpụmkpụ, na-emekarị 8 ka 11 amino asịd n'ogologo, e ji ihe nrụzi arụrụ arụ mara. Ọnọdụ 2 (P2) na ọnọdụ C-terminal (P9/P10) na-eje ozi dị ka ihe fọdụrụ arịlịka nke na-abanye n'ime obere akpa nke HLA nnukwu yinye na-ekekọta groove.. Maka alleles nkịtị dị ka HLA-A*02:01, arịlịka ndị a nwere ihe fọrọ nke nta ka ọ bụrụ nanị nke nnukwu hydrophobic aliphatic ma ọ bụ amino acid aromatic: Leucine (Leu), Valine (Val), Isoleucine (Ya na), Methionine (zutere), Phenylalanine (Phe), na Tyrosine (Taịa).
Mgbe peptides emebere emebere na-enyo enyo ndị a nwere njikọ HLA, Ọdịnaya hydrophobic ha dị elu na-eduga n'ịrụ mmiri mmiri siri ike na mgbasa ozi ngwa ngwa na-abụghị nke a kapịrị ọnụ na tubes microcentrifuge polypropylene na LC autosampler vials..
- Enweghi ike na-ebunye ihe nchekwa: Ndozigharị LC-MS ahaziri ahazi (0.1% FA / 1% ACN) ugboro ugboro na-adaghị solubilize hydrophobic 9-mers, na-eme ka mmiri ozuzo tupu ịgbanye kọlụm.
- Mfu Adsorption dị n'elu: Na picomolar ma ọ bụ femtomolar ntinye uche nke ejiri maka LC-MS spike-in calibration curves., usoro peptide na-enweghị ike ịbanye na mgbidi akpa, na-agbagọ linearity (R² < 0.95).
2. Isotopic ịdị ọcha vs. Nkwekọrịta mperi ọtụtụ
Maka nyocha nke immunopeptidomics ezubere iche na-eji nleba anya mmeghachi omume ahọpụtara (SRM), Nleba anya mmeghachi omume yiri (PRM), ma ọ bụ DIA nwere ụkpụrụ ime, A na-emepụta peptides sịntetik site na iji Stable Isotope Labeled (SIL) amino asịd. Na-emekarị, arọ Arg (13C6, 15N4; uka mgbanwe +10 Na) ma ọ bụ arọ Lys (13C6, 15N1; uka mgbanwe +8 Na) A na-etinye ya na C-terminus.
Na ngwa ndị nwere oke oke dị ka Orbitrap Astral ma ọ bụ Orbitrap Eclipse na-arụ ọrụ na 120,000 ka 240,000 ike idozi (m/z 200), A na-amachibido nnabata mmejọ ọtụtụ ka ≤ 5 ppm. Ọganihu isotopic na-ezughị ezu n'oge amino acid precursor synthesis na-emepụta "ọkụ isotope ọkụ" - ụdị peptide na-enweghị akara dị ugbu a n'ụkpụrụ akpọrọ.. Ọ bụrụ na nkwalite isotopic dara n'okpuru 99.0%, mgbama ọkụ fọdụrụnụ na-ejikọta ya na njikọ HLA dị ala dị ala, na-amụba akara nrịbama ngụpụta nke adịgboroja.
3. Ọdịnaya Peptide Net: Na-agafe gafere HPLC Ọcha anya
Echiche na-ezighi ezi na ịzụ ahịa peptide omenala na-atụle ịdị ọcha nyocha RP-HPLC (214 pasent mpaghara nm kacha elu) ya na mkpokọta peptide zuru oke.
Ịdị ọcha RP-HPLC na-egosi naanị njupụta nke peptide se lekwasịrị anya
Ọdịnaya Peptide Net (%) = Mass nke Peptide dị ọcha Src Peptides Laboratory Usoro/Ngụkọta ibu ntụ ntụ ntụ × 100
s, dị ka mmiri fọdụrụnụ, counter-ions (trifluoroacetate ma ọ bụ acetate), na agbụ nnu. Nnukwu ibu arọ gravimetric na-elekarị ọdịnaya peptide n'ezie anya site na 15% ka 35%.
Ọdịnaya Peptide Net (%) = Mass nke Usoro Peptide Dị Ọcha/Mkpokọta Lyophilized Powder arọ × 100
Maka usoro immunopeptidomics ahaziri ahazi na-achọ ọnụọgụ zuru oke (fmol/µL spike-ins), ịdabere na oke akọrọ na-ewebata usoro qu
Ọkọlọtọ siri ike-Ọkwa Peptide Synthesis (SPSS) A na-eji trifluoroacetic acid (TFA) maka nchapu site na resin na ntughari-ọcha ọcha. TFA counter-ions fọdụrụnụ dị ka nnu trifluoroacetate nwere ike igbochi ionization electrospray. (ESI) arụmọrụ n'oge Nyocha 1 Onye na-eweta Peptides LC-MS nyocha. Ọzọkwa, ndabere ndu mmetọ (endotoxins, DNA fọdụrụnụ nke nje bacteria, ma ọ bụ akara keratin/trypsin) ewebata n'oge nkwakọ peptide nwere ike kpuo ihe nchọpụta Orbitrap nwere mmetụta, nke na-arụ ọrụ na njedebe sub-femtomolar nke nchọpụta.
ons fọdụrụ dị ka trifluoroacetate salts nwere ike ịkwụsị electrospray ionization (ESI) arụmọrụ n'oge nyocha LC-MS. Ọzọkwa, ndabere ndu mmetọ (endotoxins, DNA fọdụrụnụ nke nje bacteria, ma ọ bụ akara keratin/trypsin) ewebata n'oge nkwakọ peptide nwere ike kpuo ihe nchọpụta Orbitrap nwere mmetụta, nke na-arụ ọrụ na njedebe sub-femtomolar nke nchọpụta.
Achọrọ njikwa ogo na ntụgharị maka ụkpụrụ akpọrọ aha dị arọ
Iji kwado mmepụta peptide omenala ya na ngwa-ebupụta dị elu, Ndị na-ere synthesis ga-emezigharị njikwa ogo ha (QC) frameworks na arụ ọrụ nnyefe ụdị.
Ọnụ ụzọ nyocha maka Immunopeptidomics - Peptides omenala dị njikere
| Oke | Ọkwa Nchọpụta Ọkọrịa | Immunopetidomics-Ọkwa Njikere | Usoro nkwado nyocha |
|---|---|---|---|
| RP-HPLC ịdị ọcha | ≥ 90% | ≥ 98% | C18 RP-HPLC (0.1% TFA / ACN gradient, 214 nm) |
| Ịdị oke ọnụ | ± 1.0 Na | ≤ 5 ppm (± 0.005 Na) | ESI-Orbitrap dị elu / MALDI-TOF MS |
| Ịba ụba Isotopic | > 95% | > 99.0% | Nyocha nkesa Tandem MS isotopic envelopu |
| Ọdịnaya Peptide Net | Atụghị (Mkpokọta nha) | Ezi uru enwetara site na AAA | Quantitative Amino Acid Analysis (AAA) |
| Asambodo solubility | Nyocha mmiri anya | Ekwenyere na 0.1% FA / 1% ACN | Centrifugation & Nyocha mpaghara kacha elu LC-MS |
| Ọkwa Endotoxin | Ejikwaghị ya | < 0.01 EU/mg | Chromogenic LAL Assay |
| Oghere nrụpụta | Ụlọ nyocha izugbe | Klas 100 Ime ụlọ ọcha nke Ultra-Sterile | ISO 14644-1 Klas 5 nlekota gburugburu ebe obibi |
Ihe ntụgharị dị mkpa na nyocha Neoantigen
Na ọrịa kansa immunotherapy nkeonwe yana mmepe ọgwụ mgbochi neoantigen, Ndị na-eme nchọpụta na-ekewa njikọ HLA endogenous na-eji ngwa dị ka Thermo Fisher Pierce MHC Class I Sample Prep Workflow ma chọpụta peptides mutated n'ime 48 ka 72 awa via ọzọ usoro algọridim.
Iji kwado neoantigens nwa akwukwo, ndị na-eme nchọpụta ga-emerịrị ịtụ ngwa ngwa sịntetik dị arọ nke akpọrọ (SIL) ụlọ akwụkwọ peptide (na-emekarị 20 ka 96 usoro) maka nkwado PRM. Omenala omenala peptide ndu oge nke 4 ka 6 izu na-emepụta ihe mgbochi na-adịghị anabata na pipeline nyocha ụlọ ọgwụ. Ndị na-ere peptide omenala nke oge a ga-ewepụtarịrị njikọ 96-nke ọma yana a 5 ka 7 mgbanwe ụbọchị azụmahịa na-emebighị ọnụ ụzọ QC nyocha.
Ndozi usoro maka ndị na-enye Peptide omenala: Na-eme ka nnabata onye nyocha
Iji nyere ndị nyocha aka ịnakwere ngwa immunopeptidomics ọkọlọtọ na ntụkwasị obi zuru oke, Ndị na-enye njikọ peptide omenala ga-agbanwerịrị site na ndị nrụpụta nkwekọrịta na-agafe agafe gaa na ndị mmekọ bioanalytical pụrụ iche.
Ndị na-eduga njikọ nyiwe, dị ka MOL na-agbanwe' ikpo okwu njikọ peptide pụrụ iche, gosi ka mgbanwe teknụzụ ezubere iche si edozi ihe ịma aka mgbago elu.
Nkwekọrịta atọ bara uru maka ndị na-enye PEPTIDE
- Immunopeptidomics echekwabara-katalọgụ dị njikere maka ọba akwụkwọ ntụnyere Allele akọwapụtara. (HLA-A02:01, HLA-A24:02, HLA-B*0:02) Ụkpụrụ nhazi oge njide (Ngwakọta ndị akpọrọ PRTC-ọkwa isotopically)
- Ezubere iche maka Immunopeptidomics QC Panels High-Res ESI-MS Mass Accuracy (<= 5 ppm) & Ịba ụba Isotopic (> 99.0%) Asambodo ọdịnaya AAA Net Peptide & 1% Nnwale Solubility TFA
- Ngosipụta akara SIL ngwa ngwa & Ọdịnaya Synthesis High-Troughput Parallel 96-Well SPPS (5-7 Window nnyefe ụbọchị) Klas 100 Imepụta ime ụlọ dị ọcha iji kpochapụ MS Background Mmetọ
1. Immunopeptidomics echekwabara-katalọgụ dị njikere
Ndị na-enye peptide kwesịrị inye ndị emebere mbụ, Ọbá akwụkwọ peptide enyochagoro mbụ nke ahaziri ka ọ bụrụ nnukwu ụdị HLA n'akụkụ ngwakọta nhazi oge njide..
- Ụlọ akwụkwọ ntụaka akọwapụtara nke Allele: Ngwakọta njikọ endogenous nwere njikọ dị elu maka alleles mmadụ juru ebe niile (eg., HLA-A*02:01, HLA-A*24:02, HLA-B*0:02, na HLA-DRB1*01:01). Ndị a na-eje ozi dị ka njikwa dị mma maka nrụpụta ngwa ngwa immunoaffinity.
- Ngwakọta nhazi oge njide: Cocktails peptide nwere nnukwu akara na-agbasa ụdị ụdịdị dị iche iche nke hydrophobicity (oge njigide nwayọọ nwayọọ site na 5% ka 65% ACN). Agbabanyere n'ime ngwa elu tupu ihicha C18, Ụkpụrụ ndị a na-enye ohere nhazi oge njigide akpaaka n'ofe ọtụtụ LC-MS na-agba ọsọ.
2. Ezubere iche Immunopeptidomics QC Panel
Kama ibuga Asambodo Analysis ọkọlọtọ (CoAs) nwere nkiti HPLC trace overlays, Ndị na-enye peptide ga-ewebata panel LC-MS QC pụrụ iche:
Ọnụ ego nke Immunopeptidomic CoA Standard: Peptide ọ bụla emepụtara gụnyere ESI-MS nha nha nha ziri ezi (≤ 5 ppm), tandem MS isotopic nkwalite nkwalite (> 99.0%), Ntinye peptide net nke AAA kwadoro, na nkwado solubility na 0.1% FA / 1% ACN.
Site n'iji ike nnukwu isotope akara (SIL) ụkpụrụ peptide akwadoro site na ọdịnaya net AAA akwadoro, Ndị na-eme nchọpụta na-ewepụ mkparị ịta ahụhụ mgbe ha na-eguzobe usoro nhazi nha nha zuru oke na nyiwe Orbitrap LC-MS..
3. Ndenye aha Isotope dị arọ & Synthesis ime ụlọ dị ọcha
Usoro peptide ahaziri ahazi ga-ejikọrịrị ngịkọ siri ike-Phase Peptide synthesis. (SPSS) nwere isotope kwụsiri ike nke akpọrọ amino acid (^{13}C₆, ^{15}N₄-Arg na ^{13}C₆, ^{15}Ndepụta N₁).
Ọzọkwa, iji gbochie mmetọ gburugburu ebe obibi ka ọ ghara itinye aka na nchọpụta ligand HLA dị ala, njikọ, ime ka ọ dị ọcha, na aliquoting kwesịrị ime n'ime ebe nrụpụta adịghị mma. Ụlọ ọrụ na-eji a Klas 100 gburugburu ime ụlọ dị ọcha yana njikwa mma LC-MS gbaara agba hụ na nkwụsị ion ndabere efu na ọkwa endotoxin dị ala (< 0.01 EU/mg) dabara adaba maka nyocha mmalite cellular T-cell.
Ndepụta nlebanya ndị na-ere ahịa maka ngwa ngwa Peptides omenala dakọtara
Mgbe ị na-enyocha ndị na-eweta peptide omenala maka ụkpụrụ ntụaka immunopeptidomics na ọba akwụkwọ SIL., Otu ndị na-azụ ahịa bioanalytical kwesịrị iji matriks mkpebi ndị a:
| Usoro nyocha | Omenala ndị na-eweta Peptide | Onye na-ere ihe na-anabata Immunopeptidomic (MOL mgbanwe) | Ihe ize ndụ / Mmetụta na Kit Workflow |
|---|---|---|---|
| Environment Synthesis | Mepee oche / ọkọlọtọ ụlọ nyocha | Klas 100 Ime ụlọ ọcha nke Ultra-Sterile | Mkpọtụ MS dị elu & mmetọ endotoxin na nyocha cell |
| Nnwale ịdị ọcha | Mpaghara kacha elu RP-HPLC (214 nm) | Abụọ RP-HPLC & HR-ESI-MS (≤ 5 ppm) | peptides ihichapụ amaghị ama na-ebute nnyonye anya kacha elu na ọwa PRM |
| Ntọala ọnụọgụgụ | Nnukwu arọ gravimetric | Ọdịnaya Peptide Net AAA kwadoro | Ruo 35% Njehie itinye uche na mkpokọta AQUA zuru oke |
| Ịba ụba Isotopic | > 95% General SIL | > 99.0% nkwenye siri ike | Mwepu isotope dị ọkụ na-emepụta mgbama endogenous adịgboroja |
| Nkwado Solubility | Nnwale mmiri bụ isi | Nnwale echekwabara kpọmkwem (0.1% FA / 1% ACN) | Mmiri Peptide na LC autosampler vials na-akpata mfu mgbaàmà |
| Ntụgharị nnyefe | 4 ka 6 izu | 5 ka 7 ụbọchị azụmahịa (96-nke ọma panels) | Ntụle nkwado neoantigen na nhazi nlele ụlọ ọgwụ |
Ndụmọdụ atụmatụ maka ndị nyocha Immunopeptidomics
Nhazi nke nkwadebe sample mass spectrometry nke ala site na ngwa dị ka Thermo Fisher's Pierce MHC Kit bụ nnukwu nzọụkwụ na-aga n'ihu maka nyocha proteomics.. Agbanyeghị, ihe ịga nke ọma nyocha ka na-ejikọta ya na ịdịmma nke peptides sịntetik nke elu.
Ka iwelie nghọta nyocha na ọnụọgụ ọnụọgụgụ:
- Nye iwu Ọdịnaya Ụgbụ enwetara AAA: adaberekwala na oke ntụ ntụ lyophilized maka nnukwu akara AQUA peptide spike-ins. Chọrọ asambodo AAA iji hụ na ọnụọgụ molar ziri ezi.
- Nyochaa ọnụ ụzọ nkwalite Isotopic: Gbaa mbọ hụ na ibu peptides nwere nnukwu akara > 99.0% ịdị ọcha isotopic iji zere mgbama mgbama nke yinye ọkụ na ultra-high resolution Orbitrap mass spectrometers..
- Onye mmekọ ya na ndị na-enye njikọ aka pụrụ iche: Na-arụ ọrụ na ndị na-eweta peptide nwere ike ịmepụta usoro omenala n'ime Klas 100 gburugburu ime ụlọ dị ọcha ma na-ebuga ngwa ngwa, ụlọ akwụkwọ SIL peptide yiri ya.
Site n'ịkọwa nkọwapụta peptide omenala n'elu na ngwa nhazi ihe nleba anya ala ala, Ndị nchọpụta nwere ike iji obi ike zuru oke na-eduzi ọmụmụ ihe immunopeptidomics.
Maka ndị nchọpụta na-achọ ọba akwụkwọ peptide omenala dakọtara ngwa, nnukwu isotope akara ụkpụrụ, ma ọ bụ ezubere iche immunopeptidomics QC panels, kpọtụrụ ndị ọkachamara bioanalytical na MOL Changes iji kparịta ịdị mma nke usoro omenala yana nhọrọ njikọ ọnụ ụlọ dị ọcha.
Odee & Nleba nka nka
MOL na-enyocha Bioanalytical R&D Otu
Senior Mass Spectrometry & Ndị ọkachamara na-ahụ maka peptide synthesis
Edere ma nyochaa nyocha a site na bioanalytical R&D ndị ọkachamara na MOL mgbanwe, na-ejikọta nka na nhazi peptide omenala omenala spectrometry-ọkwa, akara isotope kwụsiri ike, na mmepụta ụlọ dị ọcha maka nyocha nke immunopeptidomis ziri ezi.
