Chemistry Bioorthogonal, dị ka a vasatail usoro maka nhọrọ mgbanwe nke biomolecules n'okpuru nwayọọ physiological ọnọdụ, adọtala uche nke ndị ọkà mmụta sayensị n'afọ ndị na-adịbeghị anya n'ihi ọtụtụ ngwa ngwa ya na mmetụta dị ukwuu. Agbanyeghị, otu mmeghachi omume (eg., alkynyl, azide, tetrazine, wdg.) N'ụdị kemịkalụ pịa, dị oke ụkọ na biomolecules eke, ime ka iwebata otu ndị a bụrụ ọrụ siri ike. Cystine (Cys) A na-enwekarị protein na peptides, na a na-eme atụmatụ na banyere 2% nke cysteine (Cys) dị na ngwaahịa eke.Amino acid a, nke bara ụba na protein ma nwee ọtụtụ thiol, na-enye saịtị SH maka mgbanwe nke peptides na protein, dabere na atụmatụ mgbanwe kemịkalụ orthogonal dabere na nnu alkyl thiophenium nke ụlọ ọrụ nyocha mepụtara..
Ihe ngosi nka
- Nhọrọ nke ukwuu: Alkyl thiophenium salts na-enwe ike ozugbo na họrọ orthogonally gbanwee cysteine residues site na mmeghachi omume S_N2., usoro nke na-eme n'okpuru ọnọdụ dị nro na elu chemoselectivity na ndakọrịta.
- Multifunctional mgbanwe: Nkà na ụzụ nwere ike iwebata ọtụtụ ụdị arụ ọrụ, gụnyere agbụ PEG, fluorescent ụmụ irighiri ihe, ọgwụ ọjọọ, carbohydrates, carbohydrates, na peptides, na-eme ka ọrụ biomolecules dịkwuo mma.
- Multifunctional biocoupling: Ọmụmụ ihe ahụ na-ekpughe na teknụzụ na-enyere aka biocoupling multifunctional site na iwebata "aka" clickable dị ka alkynes na azides.. Nke a pụtara na enwere ike ịgbakọta ụdị biocouplings dị mgbagwoju anya na “nzọụkwụ abụọ., mmeghachi omume otu ite”., na-agbasawanye ohere nke ngwa biomodification.
- Ngwa na-aga nke ọma na protein: A kwadokwara itinye teknụzụ a na protein dị mgbagwoju anya. Ndị ọkà mmụta sayensị nwere ihe ịga nke ọma n'ịwapụta albumin serum bovine (BSA) na-eji alkylthioindigo salts, na-egosipụta eluigwe na ala na ịdị irè nke usoro na biomolecules mgbagwoju anya.
Usoro biocoupling orthogonal dabere na nnu alkylthiophenium bụ usoro mgbanwe dị mma na nke ziri ezi., nke na-enye ngwá ọrụ dị ike maka ịrụ ọrụ nke ọma na mgbanwe kpọmkwem nke peptides na protein, ma nwee atụmanya ngwa sara mbara.
