1. Enter a DNA or RNA sequence. The left end is 5', the right end is 3'.
2. The software translates from the 5' end starting at base 1 to get 5'-3' frame 1; from base 2 to get 5'-3' frame 2; from base 3 to get 5'-3' frame 3; from the 3' end starting at base 1 to get 3'-5' frame 1; from base 2 to get 3'-5' frame 2; from base 3 to get 3'-5' frame 3.
3. If a translated sequence contains *, the * denotes a stop codon.
2. The software translates from the 5' end starting at base 1 to get 5'-3' frame 1; from base 2 to get 5'-3' frame 2; from base 3 to get 5'-3' frame 3; from the 3' end starting at base 1 to get 3'-5' frame 1; from base 2 to get 3'-5' frame 2; from base 3 to get 3'-5' frame 3.
3. If a translated sequence contains *, the * denotes a stop codon.
1. This software is intended only for impurity analysis of linear peptides and sequences with common modifications.
2. To use it, enter the peptide sequence, solid-phase resin type, synthesis method, whether acetic anhydride capping was performed, and related information.
3. If capping was performed, enter which amino acid coupling step the capping was applied to. For example, for the sequence Ala1-Cys2-Asp3-..., if capping was performed when coupling Asp, enter 3; for multiple positions, separate them with commas.
4. For sequences containing side chains, the main chain is assumed to be coupled first followed by the side chains, and capping impurity analysis is not performed on the side chains.
5. Then enter the average molecular weight of the target impurity (multiple values allowed) and select whether the MS instrument is high-resolution or low-resolution; the software automatically analyzes the cause of impurity formation.
6. Selecting high-resolution mass spec gives a smaller MS error range and more precise results.
7. For the impurities identified, researchers should confirm again the likelihood of their formation.
8. If the software identifies multiple possible impurity causes, researchers should judge again based on the actual situation.
2. To use it, enter the peptide sequence, solid-phase resin type, synthesis method, whether acetic anhydride capping was performed, and related information.
3. If capping was performed, enter which amino acid coupling step the capping was applied to. For example, for the sequence Ala1-Cys2-Asp3-..., if capping was performed when coupling Asp, enter 3; for multiple positions, separate them with commas.
4. For sequences containing side chains, the main chain is assumed to be coupled first followed by the side chains, and capping impurity analysis is not performed on the side chains.
5. Then enter the average molecular weight of the target impurity (multiple values allowed) and select whether the MS instrument is high-resolution or low-resolution; the software automatically analyzes the cause of impurity formation.
6. Selecting high-resolution mass spec gives a smaller MS error range and more precise results.
7. For the impurities identified, researchers should confirm again the likelihood of their formation.
8. If the software identifies multiple possible impurity causes, researchers should judge again based on the actual situation.
Note: All calculations run on this server. No third-party API is called and your sequence data is never sent to any third party. Synthesis/purification difficulty, impurity analysis and screening matrices are predicted reference values, for R&D reference only.