Khemistri ea bioorthogonal, e le mokhoa o feto-fetohang bakeng sa phetoho e khethollang ea li-biomolecules tlas'a maemo a bonolo a fisioloji, e hohetse tlhokomelo ea sechaba sa mahlale lilemong tsa morao tjena ka lebaka la mefuta e mengata ea ts'ebeliso le tšusumetso e kholo. Leha ho le joalo, lihlopha tse sebetsang (mohlala, alkynyl, azide, tetrazine, etc.) ka k'hemistri e tloaelehileng ea ho tobetsa ha e fumanehe haholo ho li-biomolecules tsa tlhaho, ho etsa hore ho kenya dihlopha tsena e be mosebetsi o boima. Cysteine ea tlhaho (Cys) e fumaneha haholo ho li-peptide le liprotheine, 'me ho hakanngoa hore hoo e ka bang 2% ka cysteine (Cys) e teng lihlahisoa tsa tlhaho.Amino acid ena, e batlang e le ngata ka diprotheine mme e ruile ka dihlopha tsa thiol, e fana ka sebaka sa SH bakeng sa phetoho ea li-peptide le liprotheine, ho latela hore na leano la ho fetola lik'hemik'hale tsa orthogonal tse thehiloeng ho letsoai la alkyl thiophenium le entsoe ke mokhatlo oa lipatlisiso..
Lintlha-khōlō Tsa Theknoloji
- E khetha haholo: Matsoai a Alkyl thiophenium a khona ho fetola masala a cysteine ka kotloloho le ka mokhoa o ikhethileng ka karabelo ea S_N2., ts'ebetso e etsahalang tlas'a maemo a bonolo a nang le chemoselectivity e phahameng le ho lumellana.
- Phetoho ea mesebetsi e mengata: Theknoloji e khona ho hlahisa mefuta e mengata ea mekhoa e sebetsang, ho kenyeletsa le liketane tsa PEG, limolek'hule tsa fluorescent, lithethefatsi, lik'haboraborane, lik'habohaedreite, le li-peptides, e ntlafatsa haholo ts'ebetso ea li-biomolecules.
- Multifunctional biocoupling: Boithuto bona bo boetse bo senola hore theknoloji e nolofalletsa biocoupling e mengata e sebetsang ka ho hlahisa "li-handles" tse tobehang joalo ka alkynes le azides.. Sena se bolela hore mefuta e fapaneng ea li-biocouplings tse rarahaneng li ka bokelloa habonolo ka "mehato e 'meli., karabelo ya pitsa e le nngwe, ka ho pharalla le ho eketsa boholo ba lits'ebetso tsa biomodification.
- Tšebeliso e atlehileng ho liprotheine: Tšebeliso ea theknoloji ena ho liprotheine tse rarahaneng le eona e netefalitsoe. Bo-rasaense ba atlehile ho tšoaea bovine serum albumin (BSA) ho sebelisa letsoai la alkylthioindigo, ho bonts'a bokahohle le katleho ea mokhoa oa li-biomolecules tse rarahaneng.
Mokhoa oa orthogonal biocoupling o thehiloeng ho matsoai a alkylthiophenium ke mokhoa o sebetsang hantle le o nepahetseng oa phetoho., e fanang ka sesebelisoa se matla bakeng sa phetoho e sebetsang le e khethehileng ea li-peptide le liprotheine, mme e na le tebello e batsi ya tshebediso.
