Peptide synthesis ke theknoloji ea bohlokoa ea ho fumana li-peptide tse nang le tatellano le mesebetsi e itseng, e sebelisoang haholo mafapheng a biomedicine le mahlale a lintho tse bonahalang. Mokhoa oa setso oa motsoako oa mokelikeli o boima ebile o fana ka chai e tlase, eo ho leng thata ho phethahatsa tlhoko e ntseng e eketseha, mme e fetotswe ke Bruce Merrifield's Solid Phase Peptide Synthesis (SPSS) ho 1963, eo ka eona a ileng a fuoa Khau ea Nobel ea Khemistri ka 1984. Leha ho le joalo, mokhoa o tloaelehileng oa ho kopanya mohato o tiileng o hloka mehato e pheta-phetoang ea tšireletso, ho kopanya le ho hlatsoa ho atolosa butle-butle ketane ea peptide, 'me qetellong a khaola molek'hule e reretsoeng ho tloha ho mojari, e sa lumellaneng le mohopolo oa k'hemistri e tala. Reverse Phase Solid Phase Peptide Synthesis (RP-SPPS) e sebelisa likoloi tsa hydrophobic (mohlala, resin ea polystyrene) le metsoako ea polar (mohlala, DMF), e leng kgahlanong le tlhophiso ya mokgahlelo o tiileng wa mokgahlelo. Lilemong tsa morao tjena, RP-SPPS e 'nile ea sebelisoa haholo le ho feta tšimong ea peptide synthesis ka lebaka la melemo ea eona e ikhethang..
Melemo
Ha ho bapisoa le mokhoa o tloaelehileng oa pele o tiileng-phase synthesis, kgutlisetsa mothati o tiileng (RP-SPPS) e na le melemo ea eona e ikhethang.
Etsisa ka ho feletseng motsoako oa peptide 'meleng: E lumellana haholo le tataiso ea peptide synthesis 'meleng, e fanang ka motheo oa liteko oa in vitro bakeng sa boithuto ba peptide le protheine synthesis 'meleng.
Ho hloekisa habonolo: Sesebelisoa sa hydrophobic se bonolo ho hlatsoa ka li-solvents tsa organic, ka katleho e tlosa li-reagents tse feteletseng le lihlahisoa, le ho nolofatsa mehato ea tlhoekiso.
Phetoho e ikhethang ea carboxy-terminal: RP-SPPS e nolofalletsa phetoho ea carboxy-terminal hore e kopane ka ho toba holim'a resin e tiileng., ho felisa tlhoko ea ho fetoloa kapa ho fetoloa pele ka mor'a peptide chain synthesis.
Tse ling tse tsamaellanang le k'hemistri e tala: Reversed-phase solid-phase synthesis e hanana le karabelo tlas'a maemo a metsi 'me e hloka mokelikeli o monyane oa organic ho phethela khokahanyo ka nepo..
E loketse maqheka a sa sireletsoeng: RP-SPPS e loketse mekhoa e mengata e sa sireletsoeng ea peptide ea maiketsetso 'marakeng, ho etsa hore peptide synthesis e be bonolo.
Katoloso ea ketane ea Peptide ka lehlakoreng la N-C
Melemo ea Kopo
Ka nts'etsopele ea bajari ba bacha, ho kopanya li-reagents le li-synthesizer tse ikemetseng, RP-SPPS e tla bapala karolo e kholo tšimong ea peptide synthesis, ho fana ka litharollo tse sebetsang hantle le tse bonolo tsa peptide bakeng sa masimo a biomedicine le mahlale a lintho tse bonahalang.
